Increases in atmospheric
CO
2
partial pressure have lowered seawater
pH
in marine ecosystems, a process called ocean acidification (
OA
). The effects of
OA
during the critical stages of larval development may have disastrous consequences for some marine species, including
Babylonia areolata
(Link 1807), a commercially important sea snail in China and South East Asia. To investigate how
OA
affects the proteome of
Babylonia areolata
, here we used label‐free proteomics to study protein changes in response to acidified (
pH
7.6) or ambient seawater (
pH
8.1) during three larvae developmental stages of
B. areolata
, namely, the veliger larvae before attachment (E1), veliger larvae after attachment (E2), and carnivorous juvenile snail (E3). In total, we identified 720 proteins. This result suggested that acidification seriously affects late veliger stage after attachment (E2). Further examination of the roles of differentially expressed proteins, which include glutaredoxin, heat‐shock protein 70, thioredoxin, catalase, cytochrome‐c‐oxidase, peroxiredoxin 6, troponin T, CaM kinase
II
alpha, proteasome subunit N3 and cathepsin L, will be important for understanding the molecular mechanisms underlying
pH
reduction.
Our aim was to improve sampling of hemolymph from the snail Babylonia areolata in order to evaluate the physiological and immune capacities of hemocytes in aquaculture. We also identified appropriate types of hemolymph anti-coagulants for B. areolata. Hemolymph samples were collected using an improved foot plantaris puncture method. We screened five types of anti-coagulants from Penaeid shrimp (A), marine decapods (B), abalone (C), and oyster (D), as well as a home-made anti-coagulant (E), to act against Babylonia areolata hemocytes on the basis of cell death rate and hemocyte aggregation. We improved the former foot plantaris puncture method and identified the home-made anti-coagulant as the best anti-coagulant from the five which we tested.
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