Background: To investigate the correlation between blood lipids and the prognosis of idiopathic sudden sensorineural hearing loss (ISSNHL).Methods: We included 232 patients with ISSNHL at the Second Affiliated Hospital of Shanghai University from June 2015 to March 2017 using a prospective cohort study design. We collected information including age, gender, hypertension, diabetes, mellitus, vertigo, as well as the levels of blood total cholesterol (TC), triglycerides (TG), and low-density lipoproteins (LDL-C). We also recorded the ratio between the levels of low-density lipoproteins and the levels of high-density lipoproteins (LDL-C/HDL-C ratio). Correlations between the prognosis of ISSNHL and TC, TG, LDL-C, and LDL-C/HDL-C ratio were analyzed by univariable and multivariable logistic regression analyses.

Background. Thyroid cancer is the most common endocrine malignancy, with a recent global increase of 20% in age-related incidence. Ultrasonography and ultrasonography-guided fine-needle aspiration biopsy (FNAB) are the most widely used diagnostic tests for thyroid nodules; however, it is estimated that up to 25% of thyroid biopsies are cytologically inconclusive. Molecular markers can help guide patient-oriented and targeted treatment of thyroid nodules and thyroid cancer. Methods. Datasets related to papillary thyroid cancer (PTC) or thyroid carcinoma (GSE129562, GSE3678, GSE54958, GSE138042, and GSE124653) were downloaded from the GEO database and analysed using the Limma package of R software. For functional enrichment analysis, the Kyoto Encyclopedia of Genes and Genomes pathway analysis and Gene Ontology were applied to differentially expressed genes (DEGs) using the Metascape website. A protein-protein interaction (PPI) network was built from the STRING database. Gene expression, protein expression, immunohistochemistry, and potential functional gene survival were analysed using the GEPIA website, the Human Protein Atlas website, and the UALCAN website. Potential target miRNAs were predicted using the miRDB and Starbase datasets. Results. We found 219 upregulated and 310 downregulated DEGs, with a cut-off of p < 0.01 and ∣ log   FC ∣ > 1.5 . The DEGs in papillary thyroid cancer were mainly enriched in extracellular structural organisation. At the intersection of the PPI network and Metascape MCODEs, the hub genes in common were identified as FN1, APOE, CLU, and SDC2. In the targeted regulation network of miRNA-mRNA, the hsa-miR-424-5p was found to synchronously modulate two hub genes. Survival analysis showed that patients with high expression of CLU and APOE had better prognosis. Conclusions. CLU and APOE are involved in the molecular mechanism of papillary thyroid cancer. The hsa-miR-424-5p might have the potential to reverse the processes of papillary thyroid cancer by modulating the hub genes. These are potential targets for the treatment of patients with papillary thyroid cancer.

Background: Intra-articular disorders (ID) or anterior and/or medial displacement of the temporomandibular joint disorder (TMJ) disc are the most common form of TMJ dysfunction (TMD). TMD causes changes in the friction coefficient during TMJ movement. Herein, we provided a three-dimensional (3D) finite-elements model (FEM) including the maxilla, disc, and mandible and evaluated the stress distribution with different friction coefficient. Methods: Fourteen volunteers without TMD and 20 patients with MRI-diagnosed TMD were selected. CT and MRI data were collected to build the 3D FEA model of the mandible and TMJ disc. Stress distribution with different friction coefficient was measured. Result: In the normal model, stress distribution on the TMJ disc was 2.07 ± 0.17, 1.49 ± 0.14, and 1.41 ± 0.14 MPa with 0.001, 0.3, and 0.4 friction coefficient, respectively. In the TMD model, stress distribution was 3.87 ± 0.15, 7.23 ± 0.22, and 7.77 ± 0.19 MPa respectively. Conclusion: When the friction coefficient of the side with anterior displacement increased, stress on the disc, condyle and mandible of the opposite side increased. Simultaneously, stress values of the disc, condyle and mandible were higher than those of the normal lateral joint.

through droplets 1 or 2 days before the initial skin eruption (3). Varicella mainly occurs in children under the age of 10 years in whom it presents as a blister pruritus rash which begins on the trunk and gradually extends to the head, face, and limbs. There are well-formed, contagious viruses in skin vesicles, which can form aerosols and transmit VZV to others who have not been previously infected with VZV. Concomitant symptoms include malaise, fever, fatigue, and loss of appetite, and the illness usually lasts for one to two weeks although complications including secondary bacterial infection, pneumonia, encephalitis, hepatitis, and Raynaud's syndrome can occur. Adults and patients with underlying diseases are more likely to have more serious complications than children (4,5), including several neurological complications (6-8). We report a case of a previously healthy immunocompetent 38-year-old man with sudden deafness as the initial symptom of varicella. This kind of cases are rare and suggest the relationship between viral infection and sudden deafness.We present the following article in accordance with the CARE reporting checklist (available at http://dx.doi.

Background: Long non-coding RNA (lncRNA) LUCAT1 has recently been recognized as an oncogene in several malignancies. This study was launched to probe its role in thyroid carcinoma (TC) development and the implicated molecules. Methods: LUCAT1 expression in TC cell lines and in normal thyroid follicular epithelial cell line Nthy-ori3-1 was determined by RT-qPCR. Binding relationships between LUCAT1 and microRNA (miR)-493, and between miR-493 and a disintegrin and metalloproteinase-10 (ADAM10) were predicted on a bioinformatics system and then validated through luciferase reporter gene assays. Expression of miR-493 and ADAM10 in TC cells was determined. Gain-and loss-of functions of LUCAT1, miR-493 and ADAM10 were performed to explore their influences on the behaviors of TC cells. Xenograft tumors were induced in nude mice for in vivo studies. Results: LUCAT1 and ADAM10 were highly expressed, while miR-493 was poorly expressed in TC cell lines. LUCAT1 served as a miR-493 sponge to upregulate ADAM10 expression. Silencing of LUCAT1 discouraged proliferation, invasion, and migration but triggered apoptosis of TC cells. By contrast, these changes were abrogated by further miR-493 inhibition or ADAM10 upregulation. The in vitro experiment results were reproduced in vivo. In addition, miR-493 inhibition or ADAM10 overexpression was found to increase the phosphorylation of STAT3 in cells. Conclusion: This study evidenced that LUCAT1 increases ADAM10 expression through sequestering miR-493, leading to JAK-STAT activation and TC cell growth and metastasis. LUCAT1 and ADAM10 may serve as therapeutic targets for TC treatment.

Background：Anterior and/or medial displacement of the temporomandibular joint disorder(TMJ) disc or intra-articular disorders( ID) is the most common form of TMJ dysfunction(TMD).TMD cause change of friction coefficient during TMJ movement. In the present study, We provided a 3D finite elements models(FEM) including the maxilla, disc and mandible and evaluated the stress distribution with different friction coefficient.Methods: 14 volunteers without TMD and 20 TMD patients,who were diagnosed by MRI, were selected.CT and MRI data were collected to build 3D FEA model of mandibular and TMJ disc.Stress distribution with different friction coefficient was measured.Result: In the normal model, stress distribution on TMJ disc was 2.07±0.17,1.49±0.14,1.41±0.14MPa with 0.001 0.3 and 0.4 friction coefficient.In TMD model,stress distribution is 3.87±0.15,7.23±0.22,7.77±0.19MPa respectively. Conclusion:When the friction coefficient of the side with anterior displacement increased, stress on the disc, condyle and mandible of the opposite side increased. Simultaneously, stress values of the disc, condyle and mandible were higher than those of the normal lateral joint.

Background：Anterior and/or medial displacement of the articular disc or intra-articular disorders( ID) is the most common form of TMJ dysfunction.In the present study,3D finite elements analysis (FEA) models including the maxilla, disc and mandible were established using 3D data registration technology.Methods：Six healthy volunteers and 20 TMD patients were selected.CT and MRI data were collected to build 3D FEA model of mandibular and TMJ disc.Stress distribution with different friction coefficient was measured.Result:Results showed that maximum stress of the lateral articular disc in the normal and pathological models. In the normal model, stress distribution was 2.21,1.56,1.49 MPa with 0.001 0.3 and 0.4 friction coefficient.In ID model,stress distribution is 3.87,7.23,7.77MPa respectively. Conclusion:When the friction coefficient of the side with anterior displacement increased, stress on the disc, condyle and mandible of the opposite side increased. Simultaneously, stress values of the disc, condyle and mandible were higher than those of the normal lateral joint.

Background： Anterior and/or medial displacement of the temporomandibular joint disorder(TMJ) disc or intra-articular disorders( ID) is the most common form of TMJ dysfunction(TMD).TMD cause change of friction coefficient during TMJ movement. In the present study, We provided a 3D finite elements models(FEM) including the maxilla, disc and mandible and evaluated the stress distribution with different friction coefficient. Methods: 14 volunteers without TMD and 20 TMD patients,who were diagnosed by MRI, were selected.CT and MRI data were collected to build 3D FEA model of mandibular and TMJ disc.Stress distribution with different friction coefficient was measured. Result: In the normal model, stress distribution on TMJ disc was 2.07±0.17,1.49±0.14,1.41±0.14MPa with 0.001 0.3 and 0.4 friction coefficient.In TMD model,stress distribution is 3.87±0.15,7.23±0.22,7.77±0.19MPa respectively. Conclusion: When the friction coefficient of the side with anterior displacement increased, stress on the disc, condyle and mandible of the opposite side increased. Simultaneously, stress values of the disc, condyle and mandible were higher than those of the normal lateral joint.