Lilii Bulbus, an edible Chinese herbal medicine, has a long history in medicine. However, research on effectively boiling Lilii Bulbus is rare. To make the more nutritious Lilii Bulbus soup, the optimized boiling process, using an alternate heating mode by decoction pot carrying a mixture of water and Chinese liquor at the ration of 9:1, was established in this study. Compared to the soup prepared by the daily process, the polysaccharide amount improved by 54%, and the total heavy metals decreased by 33.5% using the optimized boiling process. In addition, the total saponins at 34.3 μg/g were determined in the soup prepared by the optimized process. Meanwhile, the colchicine content in the boiled Lilii Bulbus soup was undetectable using the optimized process. This research performs an optimized boiling process for making Lilii Bulbus soup, and provides a reference for generating high commercial value from Lilii Bulbus soup in the future.
A novel gram-negative, aerobic, rod-shaped and non-nitrogen fixing bacterium named strain T786T was isolated from a highland barley cultivation soil in Qamdo, Tibet Autonomous Region, PR China. Strain T786T grew at 5–30℃ and pH 6.0–10.0 (optimum, 20–25℃ and pH 7.0–8.0) with 0–4% (w/v) NaCl (optimum, 0%). The 16S rRNA gene sequences of strain T786T showed the highest similarity to Neorhizobium vignae CCBAU 05176T (98.70%), followed by Neorhizobium alkalisoli CCBAU 01393T (98.47%), Neorhizobium tomejilense T17_20T (98.43%), Neorhizobium huautlense S02T (98.36%), and Neorhizobium galegae ATCC 43677T (98.00%). Phylogenetic analysis based on 16S rRNA genes indicated that strain T786T was a new member of the genus Neorhizobium. The digital DNA-DNA hybridization and average nucleotide identity values between the genome sequence of strain T786T and related strains were 20.20–20.60% and 76.64–80.01%, respectively. The genomic DNA G + C content of strain T786T was 60.2%. The major cellular fatty acids were Summed feature 8 (C18:1 ω7c or C18:1 ω6c, 51.53%), C16:0 (21.74%) and Summed feature 3 (C16:1 ω7c or C16:1 ω6c, 10.21%). The polar lipids of strain T786T were consisted of diphosphatidyl glycerol, phosphatidyl glycerol, phosphatidyl ethanolamine, phosphatidyl methyl ethanolamine, unidentified phospholipid and unidentified lipids (1–4). The isoprenoid quinones of strain T786T was consisted of Q-10. The DAP and sugar components of cell wall of strain T786T were meso-DAP and ribose, glucose, respectively. Based on phenotypic, phylogenetic, and genotypic datas, strain T786T is considered to represent a novel species of the genus Neorhizobium, for which the name Neorhizobium xiangyangense sp. nov. is proposed. The type strain is T786T (= JCM 35100T = CICC 25102T).
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