Methylation of cytosine to 5-methylcytosine (5mC) is a prevalent DNA modification found in many organisms. Sequential oxidation of 5mC by TET dioxygenases results in a cascade of additional epigenetic marks and promotes DNA demethylation in mammals1,2. However, the enzymatic activity and the function of TET homologs in diverse eukaryotes remains largely unexplored. In our study of TET homologs in the green alga Chlamydomonas reinhardtii, we have found a 5mC-modifying enzyme (CMD1) that catalyzes conjugation of a glyceryl moiety to the methyl group of 5mC through a carbon-carbon bond, resulting in two novel stereoisomeric nucleobase products. The catalytic activity of CMD1 requires Fe(II) and the integrity of its binding motif His-x-Asp (HxD), which is conserved in Fe-dependent dioxygenases3. However, unlike all previous described TET enzymes which utilize 2-oxoglutarate (2-OG) as a co-substrate4, CMD1 utilizes L-ascorbic acid (vitamin C, VC) as an essential co-substrate. VC donates the glyceryl moiety to 5mC with concurrent formation of glyoxylic acid and CO2. The VC-derived DNA modification is present in the genome of C. reinhardtii and its level decreases significantly in a CMD1 mutant strain. The fitness of CMD1 mutant cells during high light exposure is reduced. LHCSR3, a critical gene for protection of C. reinhardtii from photooxidative damage in high light, is hypermethylated and downregulated compared to wild-type cells, causing a lowered capacity for photoprotective non-photochemical quenching (NPQ). Our study thus reveals a new eukaryotic DNA base modification, which is catalyzed by a divergent TET homolog and unexpectedly derived from VC, and its role as a potential epigenetic mark that may counteract DNA methylation in the regulation of photosynthesis.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.