The engulfment adaptor phosphotyrosine‐binding domain containing 1 (GULP1) is an adaptor protein involved in the engulfment of apoptotic cells via phagocytosis. Gulp1 was first found to promote the phagocytosis of apoptotic cells by macrophages, and its role in various tissues, including neurons and ovaries, has been well studied. However, the expression and function of GULP1 in bone tissue are poorly understood. Consequently, to determine whether GULP1 plays a role in the regulation of bone remodeling in vitro and in vivo, we generated Gulp1 knockout (KO) mice. Gulp1 was expressed in bone tissue, mainly in osteoblasts, while its expression is very low in osteoclasts. Microcomputed tomography and histomorphometry analysis in 8‐week‐old male Gulp1 KO mice revealed a high bone mass in comparison with male wild‐type (WT) mice. This was a result of decreased osteoclast differentiation and function in vivo and in vitro as confirmed by a reduced actin ring and microtubule formation in osteoclasts. Gas chromatography‐mass spectrometry analysis further showed that both 17β‐estradiol (E2) and 2‐hydroxyestradiol levels, and the E2/testosterone metabolic ratio, reflecting aromatase activity, were also higher in the bone marrow of male Gulp1 KO mice than in male WT mice. Consistent with mass spectrometry analysis, aromatase enzymatic activity was significantly higher in the bone marrow of male Gulp1 KO mice. Altogether, our results suggest that GULP1 deficiency decreases the differentiation and function of osteoclasts themselves and increases sex steroid hormone‐mediated inhibition of osteoclast differentiation and function, rather than affecting osteoblasts, resulting in a high bone mass in male mice. To the best of our knowledge, this is the first study to explore the direct and indirect roles of GULP1 in bone remodeling, providing new insights into its regulation.
ABS, SHI, and OOCL have been conducting a project on full-scale measurement of hull stress of a container carrier since 2006. A Hull Stress Monitoring System (HSMS) was installed on an 8063 TEU container carrier recording hull girder loads and other navigation data. Vibratory responses of the hull girder were recorded at certain conditions, such as in the limited fetch storm waves in the Mediterranean Sea. The recorded data has been analyzed to determine the level of vibratory responses and the conditions in which they occurred. Since the vibratory response is superposed to the wave frequency component of the hull girder loads, it also affects the statistics of the maximum hull girder loads in waves. The effects of the vibratory responses in the long-term have been investigated for the dynamic Vertical Bending Moment (VBM) and bow acceleration. While the full-scale measurement provides valuable data for what actually happened in real vessel operations, the actual conditions can not be controlled, such as the wave environments or loading conditions. Hence, numerical calculation results are also desirable to investigate the vibratory responses under controlled conditions taking into account the elastic hull girder properties under exact conditions for validation purposes. ABS has been applying a time domain nonlinear wave/body interaction analysis program, NLOAD3D, for the assessment of the structural responses of vessels in large waves. The NLOAD3D program has been further developed to incorporate an elastic beam model to reproduce vibratory hull girder responses, with the simulations being carried out on motions and moments for selected notable events recorded during the voyages. Comparisons of the simulation and the measurement are presented.
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