In this study, the survival of Escherichia coli O157:H7 and non-O157 STEC serogroups of O26, O111, O103, and O145 were investigated during production and storage of yogurt. For this purpose, pathogens were individually inoculated into milk after pasteurization along with the starter culture (approximately 7.00±1.00 log10 cfu/g). After incubation at 44oC (about 180 min), yogurt samples were capped and stored at 4oC for 20 days. Pathogens were enumerated at 0, 5, 10, 15, and 20th days of storage. Lactic acid content (%) and pH of the samples were also screened. Moreover, mesophilic Lactococcus spp. and mesophilic Lactobacillus spp. were enumerated during production of yogurt.After incubation, the number of E. coli O157, O26, O103, O145, O111were 6.76±0.45, 6.64±0.53, 7.12±0.43, 6.00±1.39, 5.89±1.37 log10 cfu/g, respectively. A significant decrease was determined in all groups during the storage of yogurt samples at 4oC (p<0.05). It was detected on the 20th day of storage that the number of E. coli O157:H7 and non-O157 STEC serogroups of O103 and O145 were under the detection limit. However, STEC O26 and O111 were viable around 1.51±0.98 and 1.18±0.62 log10 cfu/g respectively. Results of the study showed that Escherichia coli O157:H7 and non-O157 STEC serogroups might pose a potential health risk during production and storage of yogurt.
Amaç: Antibiyotik direnci önemli bir sağlık problemidir ve sağlık sistemi üzerine yaptığı olumsuz etkiler gün geçtikçe artmaktadır. Bu çalışmada; toplum kaynaklı üriner sistem enfeksiyonu etkeni Escherichia coli suşlarının antibiyotiklere direnç oranları incelendi.Gereç ve Yöntem: Temmuz 2019 ile Haziran 2020 arasında; toplum kaynaklı üriner sistem enfeksiyonu etkeni olarak E. coli üremesi saptanan 494 hastanın antibiyogram sonuçları ve demografik özellikleri retrospektif olarak değerlendirildi.Bulgular: E. coli'nin antibiyotiklere gösterdiği direnç oranları, en azdan en çok olana doğru sırasıyla; amikasin (%0,4), tigesiklin (%2), imipenem (%2), meropenem (%2), sefiksim (%32), seftriakson (%29) ve trimetoprim-sülfametaksazol (%28) olarak saptandı.Sonuç: Toplumdan edinilmiş üriner sistem enfeksiyonlarında ampirik olarak; oral fosfomisin veya nitrofurantoin ya da günde tek doz intramusküler amikasin kullanılmasının akılcı olacağı, öte yandan; sefiksim, trimetoprim-sülfametaksazol, siprofloksasin ve sefuroksim kullanılırken, yüksek antibiyotik direnci nedeniyle dikkatli olunması gerektiği düşünülmektedir.
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