Stropharia rugosoannulata is a widely grown edible mushroom with a high nutritional value. S. rugosoannulata polysaccharides is one of the most important bioactive components of S. rugosoannulata and has a wide range of activities. A S. rugosoannulata polysaccharides, named SRF-3, was derived from the S. rugosoannulata extraction by freeze-thaw combine with hot water extraction method, then prepareed with DEAE-cellulose column and Sephacryl S-200 HR gel column, and its hypolipidemic activity was determined. The structural characteristics of SRF-3 were analyzed by infrared spectral scanning (FT-IR), ultra-high performance liquid chromatography (UHPLC), acid hydrolysis, methylation analysis, nuclear magnetic resonance (NMR), and Gas Chromatography-Mass Spectrometer (GC-MS). SRF-3 is composed of mannose, galactose, methyl galactose and fructose with ratios of 16, 12, 58 and 12, respectively. In addition, the average relative molecular mass of SRF-3 is approximately 24 kDa. The main chain of SRF-3 is mainly composed of repeating α-D-1,6-Galp and α-D-1,6-Me-Galp units, with branches in the O-2 position of Gal. The structure is presumed to be a mannogalactan, with a small amount of t-β-D-Manp present as a side chain. Hypolipidemic activity assay showed that SRF-3 had good antioxidant and hypolipidemic effects in vitro, suggesting that SRF-3 have potential application in reducing liver fat accumulation.
The physicochemical properties, structural compositions, and functional properties of insoluble dietary fiber (IDF) extracted from Aronia melanocarpa by acid extraction method, alkali extraction method, and enzymatic extraction method (EE) were studied. The results showed that the monosaccharide compositions of the three IDF samples were similar. The average molecular weight of E-IDF was higher than that of AC-IDF and AL-IDF. The surface morphology, crystal structure, and functional groups of IDF samples were changed by different extraction methods, but the effect on thermal stability was not significant. In addition, E-IDF possessed higher water-holding capacity, oil-holding capacity, and water swelling capacity. The nitrite ion adsorption capacity of E-IDF was higher at pH 2.0. At the same time, E-IDF exhibited higher glucose adsorption capacity (GAC) and better adsorption capacity for Cu 2+ and Pb 2+ . The results indicate that the IDF sample extracted by the EE had excellent physicochemical and functional properties and showed great potential as a functional food ingredient.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.