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The effect of parental educational status on vaccination rates can carry over to offspring, even among those who attain college student status. Programs targeting students who are employed on campus and who have never been vaccinated may be an especially effective way to increase vaccination rates, as both of these factors were significantly related to parental socioeconomic status in this study.

HBV DNA changes after TACE included reactivated, decreased and stable HBV DNA levels. Although HBV reactivation did not necessarily result in exacerbation of liver damage and most HCC patients with Child-Pugh grade A and B tolerated TACE well, careful post-procedure monitoring and managing is needed.

The non-polar a-plane (11 20) In𝑥Ga1−𝑥N alloys with different indium compositions (0.074 ≤ 𝑥 ≤ 0.555) were grown on r-plane (10 12) sapphire substrates by metalorganic chemical vapor deposition, and the indium compositions 𝑥 are estimated from x-ray diffraction measurements. The in-plane orientation of the In𝑥Ga1−𝑥N with respect to the r-plane substrate is confirmed to be [ 1100] sapphire || [11 20]In 𝑥Ga1−𝑥N and [ 1101] sapphire || [0001]In 𝑥 Ga 1−𝑥 N.The effects of substrate temperature, reactor pressure and trimethylindium input flow on the indium incorporation and growth rate are investigated. The morphology of the a-plane In𝑥Ga1−𝑥N is found to be significantly improved with the decreasing indium composition 𝑥 and growth rate. Moreover, the in-plane anisotropic structural characteristics are revealed by high resolution x-ray diffraction employing azimuthal dependence, and the degree of anisotropy decreases with the increase of indium composition.

BackgroundEpithelial ovarian cancer (EOC) is the most common ovarian malignant cancer. Circular RNA is a type of endogenous noncoding RNA and is considered as a novel regulatory molecule in the development and progression of tumors. This study investigated the expression and functions of a circular RNA, circular‐phosphoglycerate mutase 1 (circ‐PGAM1), in EOC tissues and cells.MethodsThe expression of circ‐PGAM1 and miR‐542‐3p in EOC was analyzed using quantitative RT‐PCR. Immunohistochemistry and western blot were performed to confirm the localization and expression of cell division cycle 5‐like (CDC5L) and pseudopodium enriched atypical kinase 1 (PEAK1) in EOC tissues. Cell lines (CAOV3 and OVCAR3) overexpressing or silencingcirc‐PGAM1 and miR‐542‐3p were established to explore the functions of circ‐PGAM1 and miR‐542‐3p in ovarian cancer cells. Furthermore, dual‐luciferase reporter assay was performed to study the interactions between circ‐PGAM1 and miR‐542‐3p and between miR‐542‐3p and CDC5L. CCK‐8, transwell, and flow cytometry were used to study the effect of circ‐PGAM1 and miR‐542‐3p on cell biological behaviors including proliferation, migration, invasion, and apoptosis. The interaction between CDC5L and the PEAK1 gene promoter was confirmed using chromatin immunoprecipitation (ChIP).ResultsCirc‐PGAM1 was upregulated in EOC tissues, whereas linear PGAM1 was not deregulated in EOC tissues. Silencing of circ‐PAGM1 inhibited proliferation, migration, and invasion of ovarian cancer cells and promoted cell apoptosis. MiR‐542‐3p was downregulated in EOC tissues, and miR‐542‐3p overexpression inhibited malignant progression of ovarian cancer cells. Circ‐PGAM1 directly interacted with miR‐542‐3p, with mutual negative feedback between them. CDC5L was a direct target of miR‐542‐3p and played an oncogenic role in ovarian cancer cells. Furthermore, the CDC5L protein binds directly to the PEAK1 promoter to promote its transcription. PEAK1 overexpression activated ERK1/2 and JAK2 signaling pathways and promoted malignant biological behaviors of ovarian cancer cells. Circ‐PAGM1 silencing combined with miR‐542‐3p overexpression played the greatest anticancer role in vivo.ConclusionThe circ‐PGAM1/miR‐542‐3p/CDC5L/PEAK1 pathway played an important role in the progression of ovarian cancer and might be a novel therapeutic target for ovarian cancer.