ILC appeared less responsive to chemotherapy but presented a better outcome than IDC. While new information on biological features of ILC is needed, we consider that neoadjuvant endocrine therapy in hormone receptor-positive ILC may be a more adapted approach than neoadjuvant chemotherapy.

No abstract

To elucidate further the potential of a Semliki Forest virus IL-12 genes induced tumor regression, the antiangiogenic-(SFV) vector in vivo for gene therapy, we constructed a activity of SFV-IL12 was investigated using Doppler ultravector, SFV-IL12, to transfer murine IL-12 genes into sonography (DUS). SFV-IL12 inhibited in situ neovascutumors. A single intratumoral injection of established B16 larization within the tumor, without affecting the resistance murine melanoma with SFV-IL12 resulted in a significant index of pre-existing intratumoral blood flows. In addition, inhibition of tumor growth, while injection with SFV-LacZ histological analysis of SFV-IL12-treated tumors showed had no effect. This antitumoral activity correlated with an massive tumor necrosis induced by SFV-IL12 treatment. increase of IFN␥ production, MIG and IP-10 mRNA These data indicate that SFV-IL12 inhibits tumor growth expression, both at the tumor site and at the periphery. In through its antiangiogenic activity, demonstrated for the contrast, no increase in CTL-or NK cell-mediated cytotoxic first time in vivo by DUS, and suggest that the SFV vector response could be detected, ruling out the involvement of may be a novel valuable tool in tumor gene transfer. T and NK cell cytotoxicity. To determine how the transfer of

nal intensities on T1-and T2-weighted images, contrast enhancement, relationship with adjacent fascia or tendon, secondary bone involvement, and intratumoral necrosis. In 19 cases the pathology findings were available for review and for a comparative MR-pathology study.Results. On T1-weighted images, lesions were isointense (n=3), hypointense (n=7) or slightly hyperintense to muscle (n=11). Immunohistochemical examination was performed in 17 patients. All 17 specimens showed positivity for HMB-45 antibody. In nine of 11 lesions with slightly increased signal intensity on T1-weighted images, a correlative MR imaging-pathology study was possible. All nine were positive to HMB-45 antibody. Conclusions. Clear cell sarcoma of the musculoskeletal system often has a benign-looking appearance on MR images. In up to 52% of patients, this lesion with melanocytic differentiation has slightly increased signal intensity on T1-weighted images compared with muscle. As the presence of this relative higher signal intensity on T1-weighted images is rather specific for tumors displaying melanocytic differentiation, radiologists should familiarize themselves with this rare entity and include it in their differential diagnosis when confronted with a well-defined, homogeneous, strongly enhancing mass with slightly higher signal intensity compared with muscle on native T1-weighted images. Abstract Objective. To evaluate MR imaging and pathology findings in order to define the characteristic features of clear cell sarcoma of the soft tissues (malignant melanoma of the soft parts). Design and patients. MR examinations of 21 patients with histologically proven clear cell sarcoma of the musculoskeletal system were retrospectively reviewed and assessed for shape, homogeneity, delineation, sig- Key words

Background:Ex vivo colospheres have been previously characterised as a colorectal cancer (CRC) well-rounded multicellular model, exclusively formed by carcinoma cells, and derived from fresh CRC tissue after mechanical dissociation. The ability to form colospheres was correlated with tumour aggressiveness. Their three-dimensional conformation prompted us to further investigate their potential interest as a preclinical cancer tool.Methods:Patient-derived CRC xenografts were used to produce numerous colospheres. Mechanism of formation was elucidated by confocal microscopy. Expression analysis of a panel of 64 selected cancer-related genes by real-time qRT–PCR and hierarchical clustering allowed comparison of colospheres with parent xenografts. In vitro and in vivo assays were performed for migration and chemosensitivity studies.Results:Colospheres, formed by tissue remodelling and compaction, remained viable several weeks in floating conditions, escaping anoikis through their strong cell–cell interactions. Colospheres matched the gene expression profile of the parent xenograft tissue. Colosphere-forming cells migrated in collagen I matrix and metastasised when subrenally implanted in nude mice. Besides, the colosphere responses to 5-fluorouracil and irinotecan, two standard drugs in CRC, reproduced those of the in vivo original xenografts.Conclusion:Colospheres closely mimic biological characteristics of in vivo CRC tumours. Consequently, they would be relevant ex vivo CRC models.