Introduction: Pregnant women stand as an relevant group for research about Human Herpesvirus (HHV-2) infection owing to the risk of mother-to-child transmission. Methods: Women attending in a prenatal care center were tested for HHV-2 IgM and IgG by ELISA. Quantitative PCR test was the chosen method to ascertain viremia. Results: The seroprevalence of IgG and IgM anti-HHV-2 was 20.6% and 2.2% respectively. HHV-2 viremia was found in one pregnant woman with HHV-2 IgM, leading to the assumption of primary infection. Conclusion: The significantly high prevalence of HHV-2 found and the ascertainment of primary infection in a pregnant woman underline the need for constant HHV-2 follow--up and diagnosis in order to avoid sexual transmission.
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