The aim of the present study was to characterize the effects of early growth response factor-1 DNA enzyme (EDRz) in a rat abdominal aortic aneurysm (AAA) model to determine the mechanism by which EDRz inhibits AAA and affects the formation of AAA by regulating the activity of matrix metalloproteinase (MMP)-2 and MMP-9. EDRz was transfected into the abdominal aorta of rats using the jetPRIME transfection reagent following infusion with elastase. Fluorescent microscopy, hematoxylin and eosin staining, ultrastructural analysis, reverse transcription-quantitative polymerase chain reaction, western blotting and immunohistochemical analysis were performed to characterize the response to EDRz. The EDRz group showed minimal aneurysm formation when compared with the control group, with significantly lower aortic diameter expansion (2.5±0.1 vs. 3.5±0.1 mm; P<0.05). Early growth response factor 1 (Egr-1) mRNA and protein levels were significantly decreased in the EDRz group, as expected. The decrease in Egr-1 was accompanied by decreases in the mRNA and protein levels of MMP-2 and MMP-9 (P<0.05). Transfection of the Egr-1 specific synthetic DNA enzyme EDRz significantly reduced AAA following elastase infusion in rats, at least in part due to the decreased expression of downstream MMP-2 and MMP-9.
The electric servo loading system can more fully test the stability and dynamic performance of the motor body and controller, and is widely used in the research and development of flight control systems. The traditional dual PWM converter has hysteresis characteristics. When the load power is abrupt, especially when the load power is suddenly reduced, the DC output voltage of the PWM rectifier will fluctuate. Voltage fluctuations can be suppressed by increasing the capacitance of the DC side capacitors, but an increase in the capacitance capacity causes the adjustment time of the system to become longer and slows down the response speed of the system. By analyzing the fluctuation of DC output voltage caused by sudden load change, a variable feedforward control method for iq load power is proposed, which uses the method of compensating the active current on the grid side to achieve instantaneous active power balance on both sides. The simulation results show that the method reduces the DC capacitor capacity and improves the dynamic response speed of the system.
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