Aim: To review the literatures concerning the effect of the single-implant mandibular overdenture (SIMO) on patient-reported outcome measures (PROMs) and masticatory function in the fully edentulous patients.
In article 2202611 by Cui Huang, Hongye Yang, and co-workers, the extrafibrillar demineralization concept is introduced into the construction of tissue engineering scaffolds. The extrafibrillarlydemineralized dentin facilitated mechanotransduction and osteogenic differentiation of stem cells, eventually brings about new ideas to develop hierarchical collagen-based biomaterials for bone regeneration.
Dentin is a natural extracellular matrix, but its availability in bone grafting and tissue engineering applications is underestimated due to a lack of proper treatment. In this study, the concept of extrafibrillar demineralization is introduced into the construction of dentin-derived biomaterials for bone regeneration for the first time. Calcium chelating agents with large molecular weights are used to selectively remove the extrafibrillar apatite minerals without disturbing the intrafibrillar minerals within dentin collagen, resulting in the formation of an extrafibrillarly demineralized dentin matrix (EDM). EDM with distinctive nanotopography and bone-like mechanical properties is found to significantly promote cell adhesion, migration, and osteogenic differentiation in vitro while enhancing in vivo bone healing of rat calvarial defects. The outstanding osteogenic performance of EDM is further confirmed to be related to the activation of the focal adhesion-cytoskeleton-nucleus mechanotransduction axis. Overall, this study shows that extrafibrillar demineralization of dentin has great potential to produce hierarchical collagen-based scaffolds for bone regeneration, and this facile top-down fabrication method brings about new ideas for the biomedical application of naturally derived bioactive materials.
The biological clock regulated by circadian clock genes manipulates various tooth tissues including enamel, dentin, and periodontal ligament. However, whether these genes regulate cementum regeneration remains unclear. This study aims to explore whether Per1, a core gene of circadian clock genes, affects the mineralization of cementum. Immunohistochemical staining and hematoxylin-eosin staining were performed on 6-weeks-old C57BL/6 mice and showed that there were PER1-positive cells in the cementum. In addition, the expression of Per1 decreased during the mineralization of the murine cementoblast cell line OCCM-30. These findings suggest that Per1 may regulate cementum mineralization. To verify the effect of Per1 on cementum mineralization, Per1 was knocked down by lentiviral transduction in OCCM-30. The results showed that knocking down Per1 promoted the mineralization of OCCM-30, whilst it up-regulated the Wnt/β-catenin pathway. Taken together, Per1 can inhibit cementum mineralization via the Wnt/β-catenin pathway, providing a molecular foundation for the treatment of cementum loss caused by periodontal diseases.
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