Limited understanding of mitochondria disorders that induced by nanoparticles is a stumbling block for anti-cancer drug delivery targeting strategy. In present study, C6 glioma cells were exposed to aminated and alkylated SiO2 nanoparticles for mitochondrion disordering and
cell metabolism study. Collective results showed that aminated nanoparticles tend to trigger the cell-repair mechanism in cancer cells while alkylated nanoparticles could cause irreversible damages on cancer cells, although both types of the particles were proved to damage mitochondrion. The
underlying mechanism show that aminated nanoparticles induced proton-stuck effect in mitochondrion and self-repairing in cancer cells by up-regulating p21. Otherwise, alkylated nanoparticles damaged mitochondrion and induced phosphorylated cyclin E accumulation lead to Fbw7 down-regulation
caused further S phase arrest and severe late apoptosis. This work can help us elucidate the mechanism of the clinic application of nano-drug carriers.
Diverse
chains and cross-linking density of polymers are important
for cell proliferation, aging, and death. In this study, by controlling
the component ratio of N,N′-methylenebisacrylamide
(Bis)/acrylamide (Acr), we prepared polyacrylamide (PAM) hydrogels
with three different polymer structures using ultraviolet irradiation.
Moreover, we quantified their Flory’s cross-linking densities,
gel concentrations, and mechanical properties and evaluated their
influence to HL-7702 liver cell behavior and metabolism. Results showed
that PAM hydrogel at a ratio of Bis/Acr = 1:50 (Acr-50) owned the
highest cross-linking density (0.04), which provided abundant binding
sites for cell adhesion and allowed for rapid cell proliferation.
On the basis of the binding sites, cells had strong traction interaction
from fibrillate adhesion with the polymers, allowed easy cell migration,
and induced the living cell aggregations with a diameter of 800 μm.
Cells in aggregation exhibited healthy cell phenotypes and functions,
and also the mitosis of the cells in aggregation is exactly the same
with the cells in tissue. It is concluded that cell proliferation,
aging, and death can be controlled by adjusting the cross-linking
density and diverse chains of matrix hydrogels. This work will be
helpful to design new functional soft biomaterials for tissue regeneration
in the future.
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