In this study, we successfully constructed the new graphene oxide/poly-
L
-lactic acid (GO/PLLA) nanofiber scaffolds with a hydrophilic surface and porous network structure that were highly favorable for cell infiltration. When employed these new nanofiber scaffolds for a wide range of tissue engineering applications, it was expected to promote graft tissue survival and angiogenesis. The new GO/PLLA nanofiber scaffold with an appropriate concentration of 1.0 wt% was applied for the restoration of ovarian function and reserve in mice with primary ovarian insufficiency (POI). After co-transplanting the normal ovarian cortex loaded on these new nanomaterials into the in situ ovarian tissue of POI mice, the fusion of transplanted ovarian cortex with damaged ovarian tissue was improved, as well as the ovarian function and the follicle numbers. Moreover, angiogenesis was observed clearly and proved to exist in the transplanted tissue and nanomaterials, with the most conspicuous effect after co-transplantation with 1.0 wt% GO/PLLA nanofiber scaffold. In addition, nitric oxide (NO) production by phosphorylated endothelial nitric oxide synthase (p-eNOS) in vivo was proven to be involved in the effect of GO and PLLA on the improved survival rate of the transplanted ovarian cortex. This study provides a new method for the fertility preservation of ovarian tissue cryopreservation and transplantation, as well as a new strategy for the transplantation of other organs.
In order to study the interaction among the traction power supply, the train group and the operation dispatching of urban rail transit, a coupling simulation system of power supply system, trains and dispatching management is constructed. In order to solve the problems of different timescales and difficult cooperation operation for related subsystems, a multi-bus distributed real-time network architecture based on hierarchical management of communication data is established, and simulation management software is developed to facilitate the free expansion of the simulation system. Meanwhile, the track line, train operation and other large timescale subsystems are realized by the pure digital simulation. And the time-sensitive subsystems, such as train traction system, braking system, auxiliary power supply system and network system etc., are built by the semi-physical simulation. In this article, the system structure and the main implementation principle of each simulation subsystem are given in detail, and the system is tested and verified at the end. The results show that the simulation system can meet the expected requirements.
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