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Understanding which food sources are nutritionally important at nursery grounds is critical in the conservation of the dwindling populations of horseshoe crabs in Asia. We used δ 13

Seagrass meadows are among the four most productive marine ecosystems in the world. Zostera japonica (Z. japonica) is the most widely distributed species of seagrass in China. However, there is no reference genome or transcriptome available for Z. japonica, impeding progress in functional genomic and molecular ecology studies in this species. Temperature is the main factor that controls the distribution and growth of seagrass around the world, yet how seagrass responds to heat stress remains poorly understood due to the lack of genomic and transcriptomic data. In this study, we applied a combination of second- and third-generation sequencing technologies to sequence full-length transcriptomes of Z. japonica. In total, we obtained 58,134 uniform transcripts, which included 46,070 high-quality full-length transcript sequences. We identified 15,411 simple sequence repeats, 258 long non-coding RNAs and 28,038 open reading frames. Exposure to heat elicited a complex transcriptional response in genes involved in posttranslational modification, protein turnover and chaperones. Overall, our study provides the first large-scale full-length trascriptome in Zostera japonica, allowing for structural, functional and comparative genomics studies in this important seagrass species. Although previous studies have focused specifically on heat shock proteins, we found that examination of other heat stress related genes is important for studying response to heat stress in seagrass. This study provides a genetic resource for the discovery of genes related to heat stress tolerance in this species. Our transcriptome can be further utilized in future studies to understand the molecular adaptation to heat stress in Zostera japonica.

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 Understanding the temporal dynamics of seagrasses and the major influences on seagrass growth is critical for seagrass habitat conservation and administration. However, little work has been done regarding these issues in southern China. To examine inter-annual and seasonal variations of the intertidal Halophila ovalis community in southern China, we conducted quarterly sampling using the SeagrassNet methodology and assessed environmental conditions as well as direct anthropogenic impacts on the seagrass meadow from July 2008 to October 2014. Our study demonstrated strong inter-annual and seasonal dynamics of the intertidal seagrass meadow in the study area. Generally, the community performed best (highest seagrass cover, leaf area, shoot density, total biomass) in summer and worst in spring among the 4 seasons. The temporal variations in the seagrass community attributes (e.g. above-ground biomass) were significantly affected by precipitation, atmospheric visibility, and salinity, while leaf width was significantly negatively correlated with temperature, atmospheric visibility and salinity. Temperature was a major factor influencing the seagrass community (both macroalgae and seagrass), with temperature data showing an inverse relationship between seagrass and macroalgae. The aboveground: below-ground biomass ratio and leaf width of H. ovalis were the most sensitive plant parameters monitored when assessing environmental interactions. Human physical disturbances did not have a significant effect on seagrass dynamics in the study area. We concluded that long-term monitoring (like SeagrassNet) is valuable in understanding the relationship between environmental variables and seagrasses.