A marine antagonistic bacterium, JG1, was isolated from rearing water of healthy turbot (Scophthalmus maximus) in Qingdao, China. Strain JG1 was Gramnegative, straight rod and motile by polar £agella. The colony, when cultured for 24 h under room temperature, produced a yellow pigment. On the basis of morphological, physiological and biochemical characteristics, along with 16S rDNA sequence analysis, JG1 was identi¢ed as Pseudoalteromonas £avipulchra. JG1 had good inhibitory e¡ects on several bacterial pathogens of aquaculture in the genusVibrio (V. anguillarum, V. alginolyticus,V. campbellii, V. harveyi,V. mimicus, V. parahaemolyticus and V. tubiashii) and Aeromonas (A. hydrophila and A. salmonicida). No mortality occurred 14 days after zebra ¢sh and 7 days after mantis shrimp were intraperitoneally injected with JG1 at 10 6 CFU per animal, and 7 days after scallop and clam were immersed in JG1 at 10 7 CFU mL À 1 . A good antagonistic e¡ect on several bacterial pathogens and nontoxicity to the above-mentioned animals make JG1 a potential probiotic in aquaculture. In addition, a fast detection technique based on polymerase chain reaction ampli¢cation of the gyrB gene was established to allow us to determine the fate of JG1 when it is applied in aquaculture in the future.
Bone mineral density (BMD) is a complex and highly hereditary trait that can lead to osteoporotic fractures. It is estimated that BMD is mainly affected by genetic factors (about 85%). BMD has been reported to be associated with both common and rare variants, and numerous loci related to BMD have been identified by genome-wide association studies (GWAS). We systematically integrated expression quantitative trait loci (eQTL) data with GWAS summary statistical data. We mainly focused on the loci, which can affect gene expression, so Summary data-based Mendelian randomization (SMR) analysis was implemented to investigate new genes and loci associated with BMD. We identified 12,477 single-nucleotide polymorphisms (SNPs) regulating 564 genes, which are associated with BMD. The genetic mechanism we detected could make a contribution in the density of BMD in individuals and play an important role in understanding the pathophysiology of cataclasis.
RNA-Seq, a new developing high-throughput sequencing technology in recent years, provides a new and more effective method for research in the genes' expression. The technology has been used to further improve our understanding on the function of the gene structure information and excavate the new transcripts and new genes. In this study, RNA-seq was employed to study the changes of the proteins after castration in cattle. The results showed the differential expression proteins between castrated cattle and noncastrated cattle were mainly lied in immunity, lipid and fatty acid metabolism and protein synthesis. Through GO and KEGG pathway enrichment analysis, the differential expression proteins were enriched in PPAR pathway which involved in meat quality traits and lipid metabolism and immunity. In addition, the genes were mainly involved in metabolic pathways, such as ECM-receptor, interaction MAPK, signaling pathway PPAR, protein phosphorylation. The function of the proteins involved in castration were not clear and needed further researches.
Background: Syndesmotic injuries account for a significant number of ankle injuries. There is no consensus regarding the recommended method of treatment. The purpose of this study was to evaluate: functional outcomes, implant survivorship, complications, and radiographic analysis in patients who underwent fixtion with either syndesmotic screw or suture-button technique by the same experienced surgeon. Methods: This study was performed and reported in accordance with the Strengthening the Reporting of Observational studies in Epidemiology checklist. The records of 200 patients with ankle fractures who had undergone surgical treatment in our clinics between January 2014 and January 2018 were retrospectively investigated. This retrospective cohort study was approved by the institutional review board in the 2nd Hospital of Jilin University. The primary outcome measure was the American Orthopaedic Foot and Ankle Society ankle-hindfoot scale and the Foot Function Index. Secondary outcome measures included visual analog scale score, complications, range of movement of ankle, reoperations, and radiologic outcomes. For statistical comparison of the clinical and radiologic findings between the 2 groups, we used SPSS, version 21.0 (SPSS, Chicago, IL), statistical software. P Values of < .05 were considered statistically significant. Conclusion: The hypothesis was that the SB technique would achieve better functional outcomes as compared to the syndesmotic screw technique after surgery. Trial registration: This study protocol was registered in Research Registry (researchregistry5793).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.