Summarized the research status of digital holography microscopy technology. Aiming at the experimental parameters which influences the quality of hologram recurrent image, the recording and reconstruction process of hologram are analyzed, the reconstruction distance are calculated which based on the reflective off-axis digital holographic light path. The experiment records the hologram of standard resolution board and reconstruct it under different light intensity ratio. The results show that the object and reference light intensity ratio have a significant impact on the quality of hologram recurrent image. Besides, it’s the key to determining an appropriate recording distance and object and reference light intensity ratio of improving the quality of hologram. The result would provide theoretical basis and technical conditions for studying the reflective digital holography based on 3-D reconstruction.
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