In this study, a combination of an ozone gas producer and an ultrafine-bubble compressor was used to degrade tetracycline, which is a well-known antibiotic and medicine commonly used in human and animal care, and effects of varying the reaction parameters were studied. Experiments indicate that each gram of introducing ozone can degrade 2.72 g of tetracycline at pH 3 and 1.48 g at pH 11. However, basic conditions contribute to increased mineralization of tetracycline because of the ·OH radical oxidation mechanism. Higher reaction temperatures and higher ozone dosages enhance the reactivity between the ozone molecules, ·OH radicals, and tetracycline, resulting in a decline in the toxicity of the tetracycline solution as measured by cell viability. The mineralization of organic compounds is the key to decreasing the toxicity of the solution. Ultrafine-bubble ozonation can provide homogeneity of gas bubbles in solution hence it not only reduces the requirement of ozone and thus the operational cost of the reaction, but also extends the efficacy of the method to the treatment of solutions with high tetracycline concentrations.
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