During the last glacial–interglacial cycle, Arctic biotas experienced substantial climatic changes, yet the nature, extent and rate of their responses are not fully understood1–8. Here we report a large-scale environmental DNA metagenomic study of ancient plant and mammal communities, analysing 535 permafrost and lake sediment samples from across the Arctic spanning the past 50,000 years. Furthermore, we present 1,541 contemporary plant genome assemblies that were generated as reference sequences. Our study provides several insights into the long-term dynamics of the Arctic biota at the circumpolar and regional scales. Our key findings include: (1) a relatively homogeneous steppe–tundra flora dominated the Arctic during the Last Glacial Maximum, followed by regional divergence of vegetation during the Holocene epoch; (2) certain grazing animals consistently co-occurred in space and time; (3) humans appear to have been a minor factor in driving animal distributions; (4) higher effective precipitation, as well as an increase in the proportion of wetland plants, show negative effects on animal diversity; (5) the persistence of the steppe–tundra vegetation in northern Siberia enabled the late survival of several now-extinct megafauna species, including the woolly mammoth until 3.9 ± 0.2 thousand years ago (ka) and the woolly rhinoceros until 9.8 ± 0.2 ka; and (6) phylogenetic analysis of mammoth environmental DNA reveals a previously unsampled mitochondrial lineage. Our findings highlight the power of ancient environmental metagenomics analyses to advance understanding of population histories and long-term ecological dynamics.

Lake El'gygytgyn is situated in a 3.6 Ma old impact crater in northeastern Siberia. Presented here is a reconstruction of the Quaternary lake-level history as derived from sediment cores from the southern lake shelf. There, a cliff-like bench 10 m below the modern water level has been investigated. Deep-water sediments on the shelf indicate high lake levels during a warm Mid-Pleistocene period. One period with low lake level prior to Marine Oxygen Isotope Stage (MIS) 3 has been identified, followed by a period of high lake level (10 m above present). In the course of MIS 2 the lake level dropped to − 10 m. At the end of MIS 2 the bench was formed and coarse beach sedimentation occurred. Subsequently, the lake level rose rapidly to the Holocene level. Changes in water level are likely linked to climate variability. During relatively temperate periods the lake becomes free of ice in summer. Strong wave actions transport sediment parallel to the coast and towards the outlet, where the material tends to accumulate, resulting in lake level rise. During cold periods the perennial lake ice cover hampers any wave activity and pebble-transport, keeping the outlet open and causing the lake level to drop.

An accumulation terrace close to the El'gygytgyn Impact Crater in northeastern Siberia contains stratigraphic and periglacial evidence of the paleoenvironmental and paleoclimatic history and permafrost dynamics during late Quaternary time. A succession of paleo active-layer deposits that mirror environmental changes records periods favorable for the establishment and growth of ice-wedge polygonal networks and sediment variations. These two elements of the periglacial landscape serve as complementary paleoenvironmental archives that can be traced back to ∼ 14,000 cal yr BP. The slope sediments and the ground ice contained therein have prominent relative maxima and minima in properties (grain size, total organic content, oxygen isotopes). They document a regional early Holocene thermal maximum at about 9000 cal yr BP, followed by a transition to slightly cooler conditions, and a subsequent transition to slightly warmer conditions after about 4000 cal yr BP. Results from sedimentary analysis resemble morphological and geochemical (oxygen and hydrogen isotopes) results from ice wedge studies, in which successive generations of ice-wedge polygonal networks record warmer winters in late Holocene time. Moreover, peaks of light soluble cation contents and quartz-grain surface textures reveal distinct traces of cryogenic weathering. We propose a conclusive sedimentation model illustrating terrace formation in a permafrost terrain.

Between October 2008 and May 2009, the International Continental Scientific Drilling Program (ICDP) co-sponsored a campaign at Lake El´gygytgyn, located in a 3.6-Ma-old meteorite impact crater in northeastern Siberia. Drilling targets included three holes in the center of the 170-m-deep lake, utilizing the lake ice cover as a drilling platform, plus one hole close to the shore in the western lake catchment. At the lake's center. the entire 315-m-thick lake sediment succession was penetrated. The sediments lack any hiatuses (i.e., no evidence of basin glaciation or desiccation), and their composition reflects the regional climatic and environmental history with great sensitivity. Hence, the record provides the first comprehensive and widely timecontinuous insights into the evolution of the terrestrial Arctic since mid-Pliocene times. This is particularly true for the lowermost 40 meters and uppermost 150 meters of the sequence, which were drilled with almost 100% recovery and likely reflect the initial lake stage during the Pliocene and the last ~2.9 Ma, respectively. Nearly 200 meters of underlying rock were also recovered; these cores consist of an almost complete section of the various types of impact breccias including broken and fractured volcanic basement rocks and associated melt clasts. The investigation of this core sequence promises new information concerning the El´gygytgyn impact event, including the composition and nature of the meteorite, the energy released, and the shock behavior of the volcanic basement rocks. Complementary information on the regional environmental history, including the permafrost history and lake-level fluctuations, is being developed from a 142-m-long drill core recovered from the permafrost deposits in the lake catchment. This core consists of gravelly and sandy alluvial fan deposits in ice-rich permafrost, presumably comprising a discontinuous record of both Quaternary and Pliocene deposits.

Late Quaternary sediments in a permafrost environment recovered from the Elgygytgyn Impact Crater were studied to determine regional palaeoenvironmental variability and infer past water‐level changes of the crater lake. Stratigraphic analysis of a 5 m long permafrost core is based on various lithological (grain size, total organic carbon, magnetic susceptibility) and hydrochemical (oxygen isotope composition, major cation content) properties and pore ice content. The results show that alluvial sediments accumulated on top of cryogenically weathered volcanic rock. Changes in the hydrochemical properties reflect different stages of cryogenic weathering. The lithological characteristics mark the transition from an erosive site to a site with accumulation. This environmental change is linked to a relative lake level highstand at >13 000 yr BP, when a shoreline bar was formed leading to slope sedimentation. Lake level dropped by 4 m during the Holocene.

Abstract. Frozen sediments from three cores bored in the permafrost surrounding the El'gygytgyn Impact Crater Lake have been studied for pollen, non-pollen palynomorphs, plant macrofossils and rhizopods. The palynological study of these cores contributes to a higher resolution of time intervals presented in a poor temporal resolution in the lacustrine sediments; namely the Allerød and succeeding periods. Moreover, the permafrost records better reflect local environmental changes, allowing a more reliable reconstruction of the local paleoenvironments. The new data confirm that shrub tundra with dwarf birch, shrub alder and willow dominated the lake surroundings during the Allerød warming. Younger Dryas pollen assemblages reflect abrupt changes to grasssedge-herb dominated environments reflecting significantly drier and cooler climate. Low shrub tundra with dwarf birch and willow dominate the lake vicinity at the onset of the Holocene. The find of larch seeds indicate its local presence around 11 000 cal yr BP and, thus a northward shift of treeline by about 100 km during the early Holocene thermal optimum. Forest tundra with larch and shrub alder stands grew in the area during the early Holocene. After ca. 3500 cal yr BP similar-to-modern plant communities became common in the lake vicinity.

Between October 2008 and May 2009, the International Continental Scientific Drilling Program (ICDP) co-sponsored a campaign at Lake El´gygytgyn, located in a 3.6-Ma-old meteorite impact crater in northeastern Siberia. Drilling targets included three holes in the center of the 170-m-deep lake, utilizing the lake ice cover as a drilling platform, plus one hole close to the shore in the western lake catchment. At the lake’s center. the entire 315-m-thick lake sediment succession was penetrated. The sediments lack any hiatuses (i.e., no evidence of basin glaciation or desiccation), and their composition reflects the regional climatic and environmental history with great sensitivity. Hence, the record provides the first comprehensive and widely timecontinuous insights into the evolution of the terrestrial Arctic since mid-Pliocene times. This is particularly true for the lowermost 40 meters and uppermost 150 meters of the sequence, which were drilled with almost 100% recovery and likely reflect the initial lake stage during the Pliocene and the last ~2.9 Ma, respectively. Nearly 200 meters of underlying rock were also recovered; these cores consist of an almost complete section of the various types of impact breccias including broken and fractured volcanic basement rocks and associated melt clasts. The investigation of this core sequence promises new information concerning the El´gygytgyn impact event, including the composition and nature of the meteorite, the energy released, and the shock behavior of the volcanic basement rocks. Complementary information on the regional environmental history, including the permafrost history and lake-level fluctuations, is being developed from a 142-m-long drill core recovered from the permafrost deposits in the lake catchment. This core consists of gravelly and sandy alluvial fan deposits in ice-rich permafrost, presumably comprising a discontinuous record of both Quaternary and Pliocene deposits. <br><br> doi:<a href="http://dx.doi.org/10.2204/iodp.sd.11.03.2011" target="_blank">10.2204/iodp.sd.11.03.2011</a>

The sediment succession of Lake Emanda in the Yana Highlands was investigated to reconstruct the regional late Quaternary climate and environmental history. Hydro‐acoustic data obtained during a field campaign in 2017 show laminated sediments in the north‐western and deepest (up to ̃15 m) part of the lake, where a ̃6‐m‐long sediment core (Co1412) was retrieved. The sediment core was studied with a multi‐proxy approach including sedimentological and geochemical analyses. The chronology of Co1412 is based on 14C AMS dating on plant fragments from the upper 4.65 m and by extrapolation suggests a basal age of c. 57 cal. ka BP. Pronounced changes in the proxy data indicate that early Marine Isotope Stage (MIS) 3 was characterized by unstable environmental conditions associated with short‐term temperature and/or precipitation variations. This interval was followed by progressively colder and likely drier conditions during mid‐MIS 3. A lake‐level decline between 32.0 and 19.1 cal. ka BP was presumably related to increased continentality and dry conditions peaking during the Last Glacial Maximum (LGM). A subsequent rise in lake level could accordingly have been the result of increased rainfall, probably in combination with seasonally high meltwater input. A milder or wetter Lateglacial climate increased lake productivity and vegetation growth, the latter stabilizing the catchment and reducing clastic input into the lake. The Bølling‐Allerød warming, Younger Dryas cooling and Holocene Thermal Maximum (HTM) are indicated by distinct changes in the environment around Lake Emanda. Unstable, but similar‐to‐present‐day climatic and environmental conditions have persisted since c. 5 cal. ka BP. The results emphasize the highly continental setting of the study site and therefore suggest that the climate at Lake Emanda was predominantly controlled by changes in summer insolation, global sea level, and the extent of ice sheets over Eurasia, which influenced atmospheric circulation patterns.