A simple method for analyzing masses of water suspended fullerenes (nC60) in Daphnia magna by extracting to toluene and measuring by ultraviolet-vis spectrophotometry was developed. This method was used to assess bioaccumulation and depuration rates by daphnia after nC60 exposure in artificial freshwater. Accumulation was rapid during the first few hours, and based on accumulation modeling, 90% of the steady-state concentration was reached in 21 h. After exposure for 24 h to a 2 mg/L fullerene solution, the daphnia accumulated 4.5+/-0.7 g/kg wet weight, or 0.45% of the organism wet mass. Daphnids exposed to 2 mg/L fullerenes for 24 h eliminated 46 and 74% of the accumulated fullerenes after depuration in clean water for 24 and 48 h, respectively. Transmission electron microscopy revealed that the majority of the fullerenes present in the gut of daphnids were large agglomerates. The significant fullerene uptake and relatively slow depuration suggest that D. magna may play a role as a carrier of fullerene from one trophic level to another. Additionally, D. magna may impact the fate of suspended fullerene particles in aquatic ecosystems by their ability to pack fullerene agglomerates into larger particles than were found in the exposure water, and then excrete agglomerates that are not stable in water, causing them to settle out of solution. This process decreases fullerene exposure to other aquatic organisms in the water column but may increase exposure to benthic organisms in the sediment.
A key component of understanding the potential environmental risks of fullerenes (C60) is their potential effects on benthic invertebrates. Using the sediment dwelling invertebrate Chironomus riparius we explored the effects of acute (12 h and 24 h) and chronic (10 d, 15 d, and 28 d) exposures of sediment associated fullerenes. The aims of this study were to assess the impact of exposure to C60 in the sediment top layer ((0.025, 0.18 and 0.48) C60 mg/cm2) on larval growth, oxidative stress and emergence rates and to quantify larval body burdens in similarly exposed organisms. Oxidative stress localization was observed in the tissues next to the microvilli and exoskeleton through a method for identifying oxidative stress reactions generated by reactive oxygen species. Rapid intake of fullerenes was shown in acute experiments, whereas body residues decreased after chronic exposure. Transmission electron microscopy analysis revealed oxidative damage and structural changes in cells located between the lipid droplets and next to the microvilli layer in fullerene exposed samples. Fullerene associated sediments also caused changes in the emergence rate of males and females, suggesting that the cellular interactions described above or other effects from the fullerenes may influence reproduction rates.
The nonbiting midge Chironomus riparius was used to study the remediation potential and secondary effects of activated carbon (AC, ø 63-200 μm) in PCB contaminated sediments. AC amendments efficiently reduced PCB bioavailability determined by Chironomus riparius bioaccumulation tests and passive samplers. PCBs were shown to transfer from larvae to adults. Lower PCB concentrations were observed in adult midges emerging from AC amended compared to unamended sediments. Increased reproduction, survival, larval growth and gut wall microvilli length were observed with low AC dose (0.5% sediment dw) compared to unamended sediment, indicating an improved success of larvae in the sediment with low organic carbon content. On the other hand, higher AC doses (2.5% sediment dw) caused adverse effects on emergence and larval development. In addition, morphological changes in the gut wall microvilli layer were observed. This study showed that the secondary effects of AC amendments are dependent on the dose and the sediment characteristics. Metamorphic species, such as C. riparius, may act as a vector for organic pollutants from aquatic to terrestrial ecosystems and according to this study the AC amendments may reduce this transport.
HighlightsThe individual components of the biodiesel had a lower toxicity threshold than in the complex mixture.The B20 sample proved to be the most toxic for the red algae P. purpureum.The B100 sample showed the highest level of toxicity for the microalgae A. ussuriensis, C. muelleri and H. akashiwo.The sample of petroleum diesel B0 showed less toxicity compared to B20 and B100.
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