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ble HPR mRNA are regulated both developmentally and by white light (Hondred et al., 1987).We then isolated cDNA and genomic clones for a cucumber gene encoding HPR and showed that expression of the H~~ gene is li*ted to photosynthe~c organs and is regulated developmentally and by white light (Greenler et al., 1989; Hpr mRNA is Observed in etiolated cucumber cotyledons to 5 d after germh=hm (Greenler et al., 1989

A 1.9 kb clone of the T-DNA region of the Agrobacterium tumefaciens Ti plasmid Bo542 which exhibited homology to the isopentenyl transferase (ipt) locus of pTiA6 was identified by low stringency DNA hybridization. Introduction of this segment of pTiBo542 DNA into cells of Nicotiana tabacum or N. glauca caused tumor formation in vivo, and allowed hormone independent growth in vitro. Furthermore, this DNA segment complemented ipt mutant strains of A. tumefaciens, restoring their ability to cause tumors on Kalanchöe leaves and tomato stems. The complete DNA sequence of this segment has been determined, revealing an open reading frame homologous to other known Agrobacterium ipt genes.

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