CAAX proteins are subject to multiple posttranslational modifications: cysteine prenylation, CAAX proteolysis, and carboxylmethylation. For investigations of CAAX proteolysis, this study took the novel approach of using a proteolysis-resistant CAAX sequence to stage
Saccharomyces cerevisiae
Ras2 GTPase in a farnesylated and nonproteolyzed state.
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