The present study is mainly aimed at the synthesis of copper oxide nanoparticles of varied size by green synthetic approach. The structural and morphological behavior of as-synthesized CuO nanoparticles were investigated using ultraviolet-visible spectral studies (UV-Vis), Fourier transform-Infrared spectroscopy (FT-IR), X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDX), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and high-resolution transmission electron microscopy (HRTEM). The reduction of copper ions using aqueous extract of S. indica leaves produces nanoparticles of varied size and morphology. The images from SEM investigation revealed that the particles are spherical in shape with average diameter of 40–70 nm. TEM and HRTEM images clearly indicate the crystallinity and spherical nature of as-synthesized CuO nanoparticles with interplanar distance between two neighboring lattice fringes of 0.315 nm.
In the present study, we describe the facile synthesis of silver nanoparticles (AgNPs) and their nanostructures functionalized with 2-aminopyrimidine-4,6-diol (APD-AgNPs) for Hg2+ ion detection. The promising colorimetric response of APD-AgNPs to detect Hg2+ ions was visible with naked eyes and spectroscopic changes were examined by using a UV-Visible spectrophotometer. The aggregation of APD-AgNPs upon addition of Hg2+ ions was due to the chelation effect of the functionalized nanostructures and results in a color change from pale brown to deep yellow color. The probing sensitivity was observed within five minutes with a detection limit of about 0.35 µM/L. The TEM images of APD-AgNPs showed polydispersed morphologies with hexagonal, heptagonal and spherical nanostructures with an average size between 10 to 40 nm. Furthermore, the sensing behavior of APD-AgNPs towards Hg2+ ions detection was investigated using docking and interaction studies.
Helicobacter pylori (H. pylori) infection is a global public health concern. Due to its high adaptability in various adverse environments (temperature, pH, adhesion, phenotypic forms), targeting the bacterium is quite challenging. Moreover, due to its high persistence, decreased patience compliance and emerging antibiotic resistance, researchers have been forced to search for novel candidates with lesser or no side effects. Hence, in the current study, phytobioactives have been screened for its anti-biofilm attributes against H.pylori. Gastric biopsy samples have been screened using confirmatory techniques (microbiological, biochemical and molecular) for their virulent and non-virulent biomarkers. Physico-nutritive parameters were standardized. H. pylori biofilms were assessed using microtitre plate assay. Biofilms' biomass and exopolysaccharide have been evaluated using crystal violet and ruthenium red staining, respectively. Anti-biofilm screening was performed using potent aqueous phytochemicals namely Acorus calamus, Colocasia esculenta and Vitex trifolia. The results indicated the confluent growth of the H. pylori biofilms confirmed through genotyping and grew best at 37 °C for 72 h at a pH of 7.5 on polystyrene plates. Further, among the phytochemicals tested, Acorus calamus exhibited the highest H. pylori anti-biofilm activity via a dose-dependent pattern. The overall observations of the study will pave way for newer approaches to understand and combat bacterial pathogenesis and will contribute towards better health and hygiene.
The nanorods of vanadium oxide (V2O5) is synthesized by environmentally benign synthetic approach using a phytomolecule, 4‐(3‐(3,4‐dimethoxybenzyl)‐4‐methoxy‐2‐(methoxymethyl)butyl)‐3,6‐dimethoxybenzene‐1,2‐diol, isolated from Phyllanthus amarus. The rod shaped V2O5 nanostructures with the widths about 10–60 nm and the lengths reaching up to 1.5 μm was evident from scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analysis. The orthorhombic structure of V2O5 is apparent from X‐ray diffraction (XRD) results. Furthermore, the crystalline nature of V2O5 nanorods was supported by the results of high‐resolution TEM (HRTEM) and selected area electron diffraction (SAED) analysis, which showed that the interplanar distance between two lattice fringes is 0.33 nm corresponding to (001) reflection plane. The X‐ray photoelectron spectroscopic (XPS) studies revealed that the peak at 533.6 eV indicates the presence of metal oxide and also confirms that the oxidation state of vanadium in as‐prepared V2O5 is V5+. The scission of supercoiled plasmid DNA was observed from 2D gel‐image, which clearly indicated that the propensity of plasmid cleavage increases with increase in concentration of V2O5 nanorods. Interestingly, high degree of cell viability with relatively less cytotoxicity was observed for V2O5 nanorods than phytomolecule in comparison with standard drug, Methotrexate against breast cancer cell line MDA MB 231 and MCF 12 A human cell line, respectively.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.