SummaryThe relative advantages of LC-UVand LC-MS-MS methodologies for taxol as applied to toxicokinetic and pharmacokinetic studies is reviewed and some specific applications of the two methods used in our laboratories compared. The LC-MS-MS method was found to be ~enty-five times more sensitive than the LC-UV method. In essence where plasma concentrations are high and rapid turn round of data is not required the use of the LC-UV is more than adequate. However if sensitivity is an issue, limited amounts of plasma are available and/orwhere tight deadlines are pivotal LC-MS-MS is the method of choice.
Awell characterised and fullyvahdatd bioanalytical method used for measuring a novel porphyrin photodynamic therapy (Compound I) in human plasma was modified in order to enable the measurement of a structurally and metabolically related compound (Compound II) in rat plasma.The modified method was validated, but after initial attempts to implement the method to support routine sample analysis failed, it was suspected that Compound II was unstable in rat plasma.The preparation of plasma Quahty Control (QC) samples used du ring validation to evaluate precision, inaccuracy stabilily and the on-going performance of the method, was closely scrutinised because of the limited solubility of this class of compou nd in aqueous solution. After investigating the solvent composition of standard solutions of Compound II used du ring the preparation of QCs, together with the subsequent mixing techniques used to distribute the analyte in plasma, the limited solu bihty of Compound II was eventually shown to be the cause of the "stabilily" problem.
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