Biogas production can supplement the renewable energy target of the world. For this abundantly available agricultural waste like wheat and rice straw can be used. Biogas generation using this waste will curb the stubble burning incidences, reduce greenhouse gases, enhance farmer’s income, and strengthen the energy security of many countries. The recalcitrant nature of rice straw is a barrier to its hydrolysis, which is a prerequisite of the anaerobic digestion (AD) process. Alkaline, electro-hydrolysis, and a combination of both pretreatment (alkaline electrohydrolysis) methods are undertaken in the present study. Batch reactors at ambient temperature were used for AD of rice straw at different inoculums to substrate ratios (ISR) of 0.5, 0.75, and 1.0 to observe the effect on biogas/methane yield. Among these, a higher amount of biogas was obtained for ISR of 0.5 in all sets of experiments. The combined pretreatment method yielded biogas of 315.9 mL/gVS (equivalent to methane of 167.4 mL/gVS). For pretreat-ed rice straw by electro-hydrolysis, alkaline, and combined (alkaline and electro-hydrolysis) respectively, the methane yields were 7.03, 18.13, and 49.82% higher than untreated rice straw. The biogas had approximately 53% of methane content. The use of rice straw for biogas production may prove a viable alternative for clean and sustainable energy. In the studies, a first-order kinetic model is found to fit better the experimental results.
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