Abstract:Food production needs to increase to satisfy the demand due to increasing human population worldwide. To minimize this food crisis, an increase in the rice production is necessary in many countries. The current study was undertaken to evaluate the impact of Mr. Trivedi's biofield energy treatment on rice (Oryza sativa) for its growth-germination of seedling, glutathione (GSH) content in seedling and mature plants, indole acetic acid (IAA) content in shoots and roots and DNA polymorphism by random amplified polymorphic-DNA (RAPD). The sample of O. sativa cv, 644 was divided into two groups. One group was remained as untreated and coded as control, while the other group was subjected to Mr. Trivedi for biofield energy treatment and denoted as treated sample. The growth-germination of O. sativa seedling data exhibited that the biofield treated seeds was germinated faster on day 3 as compared to control (on day 5). The shoot and root length of seedling was slightly increased in the treated seeds of 10 days old with respect to untreated seeds. Moreover, the plant antioxidant i.e. GSH content in seedling and in mature plants was significantly increased by 639.26% and 56.24%, respectively as compared to untreated sample. Additionally, the plant growth regulatory constituent i.e. IAA level in root and shoot was significantly (p<0.05) increased by 106.90% and 20.35%, respectively with respect to control. Besides, the DNA fingerprinting data using RAPD, revealed that the treated sample showed an average range of 5 to 46% of DNA polymorphism as compared to control. The overall results envisaged that the biofield energy treatment on rice seeds showed a significant improvement in germination, growth of roots and shoots, GSH and IAA content in the treated sample. In conclusion, the treatment of biofield energy on rice seeds could be used as an alternative way to increase the production of rice.

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Magnesium (Mg), present in every cell of all living organisms, is an essential nutrient and primarily responsible for catalytic reaction of over 300 enzymes. The aim of present study was to evaluate the effect of biofield treatment on atomic and physical properties of magnesium powder. Magnesium powder was divided into two parts denoted as control and treatment. Control part was remained as untreated and treatment part received biofield treatment. Both control and treated magnesium samples were characterized using X-ray diffraction (XRD), surface area and particle size analyzer. XRD data showed that biofield treatment has altered the lattice parameter, unit cell volume, density, atomic weight, and nuclear charge per unit volume of treated magnesium powder, as compared to control. In addition, the crystallite size of treated magnesium was significantly reduced up to 16.70, 16.70, and 28.59% on day 7, 41 and 63 respectively as compared to control. Besides this, the surface area of treated magnesium powder was increased by 36.5 and 10.72% on day 6 and 72 respectively, whereas it was reduced by 32.77% on day 92 as compared to control. In addition, biofield treatment has also altered the particle sizes d 10 , d 50 , and d 99 (size, below which 10, 50, and 99% particles were present, respectively) as compared to control. Overall, data suggest that biofield treatment has substantially altered the atomic and physical properties of treated magnesium powder.

Staphylococcus saprophyticus (S. saprophyticus) is a Gram-positive, coagulase-negative facultative bacterium belongs to Micrococcaceae family. It is a unique uropathogen associated with uncomplicated urinary tract infections (UTIs), especially cystitis in young women. Young women are very susceptible to colonize this organism in the urinary tracts and it is spread through sexual intercourse. S. saprophyticus is the second most common pathogen after Escherichia coli causing 10-20% of all UTIs in sexually active young women [1-3]. It contains the urease enzymes that hydrolyze the urea to produce ammonia. The urease activity is the main factor for UTIs infection. Apart from urease activity it has numerous transporter systems to adjust against change in pH, osmolarity, and concentration of urea in human urine [2]. After severe infections, it causes various complications such as native valve endocarditis [4], pyelonephritis, septicemia, [5], and nephrolithiasis [6]. About 150 million people are diagnosed with UTIs each year worldwide [7]. Several virulence factors includes due to the adherence to urothelial cells by release of lipoteichoic acid is a surface-associated adhesion amphiphile [8], a hemagglutinin that binds to fibronectin and hemagglutinates sheep erythrocytes [9], a hemolysin; and production of extracellular slime are responsible for resistance properties of S. saprophyticus [1]. Based on literature, S. saprophyticus strains are susceptible to vancomycin, rifampin, gentamicin and amoxicillin-clavulanic, while resistance to other antimicrobials such as erythromycin, clindamycin, fluoroquinolones, chloramphenicol, trimethoprim/sulfamethoxazole, oxacillin, and

Methyl-2-napthylether (nerolin) is an organic compound and has the applications in pharmaceutical, and perfume industry. The stable isotope ratio analysis is increasing importance in various field of scientific research. The objective of the current study was to evaluate the effect of the biofield energy treatment on the isotopic abundance ratios of P M+1 /P M (2 H/ 1 H or 13 C/ 12 C or 17 O/ 16 O) and P M+2 /P M (18 O/ 16 O) in nerolin using the gas chromatography-mass spectrometry (GC-MS). The compound nerolin was divided into two parts-one part was control sample (untreated), and another part was considered as biofield energy treated sample which was received the biofield energy treatment through the unique biofield energy transmission process by Mr. Mahendra Kumar Trivedi (also known as The Trivedi Effect ®). The biofield energy treated nerolin was analyzed at different time intervals and were represented as T1, T2, T3, and T4 in order to understand the effect of the biofield energy treatment on isotopic abundance ratio with respect to the time. From the GC-MS spectral analysis, the presence of the molecular ion peak C 11 H 10 O + (m/z 158) along with major fragmented peaks C 10 H 7 O-(m/z 143), C 10 H 8 (m/z 128), C 9 H 7 + (m/z 115), C 7 H 5 + (m/z 89), C 5 H 3 + (m/z 63), C 4 H 3 + (m/z 51), and C 3 H 3 + (m/z 39) were observed in both control and biofield treated samples. Only, the relative peak intensities of the fragmented ions in the biofield treated nerolin was notably changed as compared to the control sample with respect to the time. The isotopic abundance ratio analysis of nerolin using GC-MS revealed that the isotopic abundance ratio of P M+1 /P M in the biofield energy treated nerolin at T1, T2, T3, and T4 was increased by 0.17, 135.83, 9.13, and 25.57%, respectively as compared to the control sample. Likewise, the isotopic abundance ratio of P M+2 /P M at T1, T2, T3, and T4 was increased by 2.38, 138.10, 13.10, and 32.14%, respectively in the biofield treated nerolin as compared to the control sample. Overall, the isotopic abundance ratios of P M+1 /P M (2 H/ 1 H or 13 C/ 12 C or 17 O/ 16 O) and P M+2 /P M (18 O/ 16 O) were significantly increased in the biofield energy treated sample as compared to the control sample with respect to the time. It is concluded that Mr. Trivedi's biofield energy treatment has the significant impact on alteration in isotopic abundance of nerolin as compared to the control sample. The biofield treated nerolin might display different altered physicochemical properties and rate of reaction and could be an important intermediate for the production of pharmaceuticals, chemicals, and perfumes in the industry.