Bambara groundnut (BG, Vigna subterranea) and moringa (Moringa oleifera) are underutilized crops that improve nutritional quality of diets locally. The objectives of this study were to measure the performance of both crops on a marginal soil, analyze the harvested plant parts and noodles produced from them, and to undertake taste testing and survey consumer reactions. The noodles contained either 100% wheat flour or wheat with either BG flour (20%) or moringa powder (6%). BG yielded 0.70 t ha −1 of dry nuts and moringa 1.54 t ha −1 of dry leaflets. Both plant products were high in nutrients (especially K, and Ca in moringa) and some amino acids. Inclusion in noodles significantly enhanced their nutritional composition particularly energy content, crude fat, crude fiber and carbohydrate. Significant increases (p < 0.05) of Mg, Mn, P, K, and Zn occurred. Total essential amino acid concentration increased from 34.1% in the pure wheat noodles to 38.2% in BG and 34.8% in moringa noodles. Sensory analysis showed acceptability of all three noodle types was above the "Neither like nor dislike" category leaning toward the liking end for almost all the attributes tested. A consumer reaction survey showed there were internal and external factors motivating respondents to choose both products. Weak correlations were found between some of these factors and respondents' willingness to pay more for the BG and moringa noodles. We conclude that adopting a comprehensive research approach from plant to plate can assist transfer of underutilized crops from field studies to acceptable consumer products with enhanced nutritional profiles.

Globally, farming systems are mostly dominated by monoculture, which has the advantage of profitability at the expense of ecological systems. Recent years have witnessed an increasing momentum in global efforts to deploy sustainable agriculture practices that mimic ecological processes, with agroecology at the forefront. In addition to the ecological aspect, agroecology also encompasses economic and social aspects targeting the whole food system. Transformative agroecology has been recognized as a stepping stone to achieving several Sustainable Development Goals (SDGs), due to its great potential to build climate change-resilient farming systems while enhancing ecosystem services and reducing biodiversity loss. Nonetheless, the available literature on the recent developments and future trajectories of the adoption of agroecology approaches for improving the production of cereals, the most important group of food crops, is limited. This review aims to highlight the blueprint of agroecology that can contribute to the achievements of the SDGs, allowing explicit interpretation of the term that will benefit twenty-first century agriculture. Using cereal crops as the case study, we provide insights into how far this field has come and the main barriers to its adoption, and conclude that this approach of “science for and with society” is the way forward for building a resilient future.

The rhizosphere microbiome is a major determinant of plant health, which can interact with the host directly and indirectly to promote or suppress productivity. Oil palm is one of the world’s most important crops, constituting over a third of global vegetable oil production. Currently there is little understanding of the oil palm microbiome and its contribution to plant health and productivity, with existing knowledge based almost entirely on culture dependent studies. We investigated the diversity and composition of the oil palm fungal microbiome in the bulk soil, rhizosphere soil, and roots of 2-, 18-, and 35-year old plantations in Selangor, Malaysia. The fungal community showed substantial variation between the plantations, accounting for 19.7% of community composition, with compartment (root, rhizosphere soil, and bulk soil), and soil properties (pH, C, N, and P) contributing 6.5 and 7.2% of community variation, respectively. Rhizosphere soil and roots supported distinct communities compared to the bulk soil, with significant enrichment of Agaricomycetes, Glomeromycetes, and Lecanoromycetes in roots. Several putative plant pathogens were abundant in roots in all the plantations, including taxa related to Prospodicola mexicana and Pleurostoma sp. The mycorrhizal status and dependency of oil palm has yet to be established, and using 18S rRNA primers we found considerable between-site variation in Glomeromycotinian community composition, accounting for 31.2% of variation. There was evidence for the selection of Glomeromycotinian communities in oil palm roots in the older plantations but compartment had a weak effect on community composition, accounting for 3.9% of variation, while soil variables accounted for 9% of community variation. While diverse Mucoromycotinian fungi were detected, they showed very low abundance and diversity within roots compared to bulk soil, and were not closely related to taxa which have been linked to fine root endophyte mycorrhizal morphology. Many of the fungal sequences showed low similarity to established genera, indicating the presence of substantial novel diversity with significance for plant health within the oil palm microbiome.

While previous research has demonstrated that multiplex polymerase chain reaction (PCR) can be a cost-effective approach to detect various genes in crops, the availability of multiplex assays to simultaneously screen both grain quality and biotic stress resistance traits in rice (Oryza sativa) is limited. In this work, we report six novel multiplex assays that use a universal protocol to detect major rice grain quality (amylose content and fragrance) and biotic stress (blast, sheath blight, and bacterial leaf blight) traits with amplified products consisting of up to four primer pairs that can be analyzed using a standard agarose-based gel electrophoresis system. Recent studies have suggested that weedy rice has novel sources of disease resistance. However, an intensive screening of weedy biotypes has not been reported in Malaysia. Accordingly, we employed one of the developed multiplex assays to screen reported genes or quantitative trait loci (QTLs) associated with blast, sheath blight, and bacterial leaf blight diseases in 100 weedy rice biotypes collected from five local fields, with phenotyping performed to validate the genotyping results. In conclusion, our universal multiplex protocol is effective for the large-scale genotyping of rice genetic resources, and it can be employed in routine molecular laboratories with limited resources.