Zoledronic acid (ZOL) is a third generation bisphosphonate which can be used as a drug for the treatment of osteoporosis and metastasis. In this study, graphene oxide (GO) is conjugated with ZOL, and the nanostructured material is evaluated in terms viability, proliferation and differentiation. Furthermore, the associated morphological changes of bone marrow-derived mesenchymal stem cells (BM-MSC), and Michigan Cancer Foundation-7 (MCF-7) breast cancer cells, as well as the effect of the drugs on mineralization of BM-MSCs are investigated using a variety of characterization techniques including Fourier Transform Infrared Spectroscopy (FTIR), scanning electron microscopy (SEM) as well as alamar blue, acridine orange, and alizarin red assays. Nanostructured ZOL-GO with an optimum performance is synthesized using ZOL and GO suspensions with the concentration of 50 µM and 2.91 ng/ml, respectively. ZOL-GO nanostructures can facilitate the mineralization of BM-MSC cells, demonstrated by the formation of clusters around the cells. The results obtained confirm the performance of ZOL-GO nanostructures as promising drug complexes for the treatment of osteoporosis and metastasis.
Graphene oxide (GO) flakes of different sizes were prepared and dispersed in isotropic and nematic (anisotropic) fluid media. The dielectric relaxation behaviour of GO-dispersions was examined for a wide temperature (25–60 oC) and frequency range (100 Hz–2 MHz). The mixtures containing GO flakes exhibited varying dielectric relaxation processes, depending on the size of the flakes and the elastic properties of the dispersant fluid. Relaxation frequencies of the GO doped isotropic media, such as isopropanol IPA, were observed to be much lower than the GO doped thermotropic nematic medium 5CB. It is anticipated that the slow relaxation frequencies (~10 kHz) could be resulting from the relaxation modes of the GO flakes while the fast relaxation frequencies (~100 kHz) could indicate strongly slowed down molecular modes of the nematogenic molecules, which are anchored to the GO flakes via dispersion interactions. The relaxation frequencies decreased as the size of the GO flakes in the isotropic solvent was increased. Polarizing microscopy showed that GO flakes with a mean diameter of 10 μm, dispersed in water, formed a lyotropic nematic liquid crystal phase. This lyotropic nematic exhibited the slowest dielectric relaxation process, with relaxation frequencies in the order of 2 kHz, as compared to the GO-isotropic suspension and the GO-doped 5CB.
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