The efficiency of lixiviate on the tolerance of plantain to root attacks of Pratylenchus coffeae has been examined. During the growing phase which lasted a month, four different treatments were applied to Banana plants of cultivars CORNE 1 and PITA 3: lix- (no lixiviate), lix100 (100% lixiviate), lix25 (25% lixiviate) and lix5 (5% lixiviate). Then, these plants were inoculated with 500 individuals of P. coffeae to assess the effect of lixiviate treatments against nematodes on both cultivars CORNE 1 and PITA 3. This study showed that 5% lixiviate and 25% lixiviate doses gave better agronomic parameters (mass of root system, the collar circumference, pseudo stem height, leaf area and a number of leaves) evaluated on vivoplants. Cultivars CORNE 1 treated with 25% lixiviate dose and cultivars PITA 3 treated with 5% lixiviate dose, gained tolerance against nematode’s attacks. Otherwise, cultivars PITA 3 were resistant to nematodes after 25% lixiviate dosage because P. coffeae were reduced by 80 % in this treatment. The results presented in this work are encouraging for the development of biological control methods of banana nematodes by lixiviate
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