Burrs were collected from paddocks on 3 properties in northern New South Wales where the age of the Trifolium subterraneum var. brachycalycinum cv. Clare swards varied from 19 to 28 years. At 1 site burrs were also sampled from swards sown 2 and 10 years previously. Twenty seedlings from these burrs and 20 plants of certified cv. Clare were grown as spaced plants in a nursery. These were assessed for vegetative and floral characters, flowering time, number of seeds per burr, seed weight, and percentage hardseed after storage at 25/25�C for 6 months and 25/45�C for a further 6 months. For most plants the mean number of days from sowing to first flower was similar to that of Clare. Compared with the naturalised strains, Clare had the lowest (P<0.05) mean number of seeds per burr: about 25% below the mean of the strains (2.7 seeds per burr). While the lowest mean seed weights of the strains were not significantly different from those of Clare, the seed weights of plants from 3 sites were higher (P<0.05) than those of Clare. After storage for either 6 or 12 months, hardseed levels were also lowest (P<0.05) for Clare. Plants from the 2-year-old sward had the same median number of seeds per burr (2.0) as Clare. As sward age increased, the median number of seeds per burr increased to 2.8. Hardseed percentages were lowest for plants of Clare and for those from the 2-year-old sward after 6 months, and for Clare after 12 months. These studies indicated the presence of divergent strains in old swards of Clare in a summer rainfall environment. Natural selection among variability within Clare is the most likely reason for the development of these strains in an environment marginal for the long-term persistence of this softseeded cultivar. Although strains had the same vegetative and floral markings as Clare, differences in ecologically important characters such as number of seeds per burr, seed weight, and hardseededness may result in plants that are better adapted to the environment in which they evolved. From these studies 23 plants of T. subterraneum var. brachycalycinum were selected for further evaluation.
The effects of spotted alfalfa aphids and blue-green aphids on the seedling survival, yield and seed production of Medicago truncatula var. truncatula cultivars Cyprus and Jemalong, M. polymorpha var. vulgaris and M. minima var. minima were investigated in field plots to which three insecticide treatments were applied: sprayed continuously to the post-flowering and haying-off stage; sprayed only until flowering ; and unsprayed. In the unsprayed plots all four medics were suitable hosts for both aphid species. The highest number of blue-green aphids developed on M. truncatula cv. Cyprus and the highest number of spotted alfalfa aphids on M. polymorpha var. vulgaris. Aphid numbers were relatively low from emergence to establishment and had no effect on the seedling mortalities of the medics in the unsprayed plots. The relatively low aphid numbers through winter also had no effect on dry matter production, but as numbers of both species increased in the spring the mean yields from all the unsprayed plots at successive harvests in September, October and November were reduced by 50, 54 and 55%, respectively. Flowering commenced in M. truncatula cv. Cyprus, M, polymorpha and M. minima before aphid numbers were high enough to reduce herbage yields in the unsprayed plots. However, M. truncatula cv. Jemalong flowered 10-1 4 days after the other medics, and its seed yields from the unsprayed plots were significantly lower than those of M. truncatula cv. Cyprus. The germination percentage of seed produced in the unsprayed plots of M, polymorpha was significantly lower than that of the other unsprayed medics.
Field experiments were conducted in a predominantly summer rainfall environment to investigate burr burial, seed production, seed characteristics, seedling emergence and survival, and the effects of time of sowing on 3 Trifolium subterraneum var. brachycalycinum lines (cv. Clare and 2 local lines). Each line produced more surface than buried burrs; surface burrs were 59% (range 56-62%) of the total number of burrs recovered and produced 59% of the total seed number. Numbers of seeds per burr were similar for surface and buried burrs, however, buried seeds were 0.97 mg heavier (P<0.05) than surface seeds. Storage for 5 months at 25/60�C decreased hardseed content of surface seed by 50% and buried seed by 70%. Surface and buried seeds stored at 25/25�C for 3 months prior to sowing in trays had a total emergence of <10%, compared with 70% emergence for seeds stored at 25/60�C before sowing. These emergence differences reflected their levels of hardseededness. Numbers of seeds recovered from the soil were not significantly different among lines, declining from about 4200 seeds/m2 after initial seed set to 150 seeds/m2 by the following winter, a 97% decrease. Seed production in the second year increased seed reserves to about 8730 seeds/m2. With no further seed production, levels had declined by 93% in June 1990 and by 99% in May 1991. These data confirm the importance of annual seed production for persistence. Total seedling emergence in summer-autumn accounted for only 10% of the estimated seed production in each year. Seedling survival in summer-autumn 1988-89 was 92.7%, more than double the survival in 1989-90. The effect of sowing time on flowering was always significant, with time to first flower being highest (196 days) for the earliest sowing in March (P<0.05), progressively decreasing (P<0.05) to 108 days for the latest sowing in July. In March, April and May sowings, inflorescence numbers on the first day of flowering were similar at about 120/m2, but increased markedly (P<0.05) for sowing in June or July. However, for the March and July sowings, number of inflorescences at the 9 November 1990 count, were lowest (P<0.05). May or June sowings had the highest number of burrs and seeds (P<0.05), indicating that these may be the best sowing times for maximum seed production in these Trifolium subterraneum var. brachycalycinum lines.
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