Medicinal plants have been the focus of several studies due to their nematicide properties which can be used to control nematodes in sheep. No study has examined the morphological effects of Cymbopogon citratus on nematodes. Thus, this study evaluated the chemical composition, nematicidal activity and effects of C. citratus extracts on the morphology of eggs and infective larvae (L3) of sheep. Aqueous and methanolic extracts and fractions of C. citratus were obtained and analysed in vitro. The C. citratus extracts were effective against Haemonchus spp. and Trichostrongylus spp. larvae and eggs. Ten fractions were obtained from C. citratus, six of which had high ovicidal activity at 1000 μg mL−1, and two fractions had high activity at all tested concentrations. The phytochemical analysis identified the presence of compounds such as terpenoids, various ketones, esters, and fatty acids. The ultrastructural analysis showed deformations of the cuticle and wilting along the body of the nematodes at all concentrations. The muscular layer, intestinal cells and the mitochondria profile showed damage compared to the typical pattern. Ultra-thin sections of eggs treated with methanolic fractions of C. citratus presented modifications. This study showed the biological activity and effects of C. citratus on the gastrointestinal nematodes in sheep.
O projeto Espaço Olericultura, localizado na Unidade de Apoio à Pesquisa UENF-Pesagro, tem como proposta desenvolver atividades de vivência e troca de experiências entre produtores, técnicos, pesquisadores, estudantes e comunidade em geral sobre o desenvolvimento do cultivo de hortaliças na região. Nesta área serão conduzidos cultivos em campo e ambiente protegido utilizando manejo e práticas culturais adequadas às condições climáticas da região. A área integrará o desenvolvimento de projetos de pesquisa, aulas e práticas de extensão nas quais as atividades e pesquisas científicas realizadas no Setor de Horticultura/UENF relacionadas à produção de hortaliças poderão ser divulgadas para o público através de cursos, dias de campo e palestras. Espera-se que o acesso à informação técnica resultante de pesquisas voltadas para as condições regionais permitam àqueles que desejam ou já praticam a produção de hortaliças possam desenvolvê-la de forma eficiente. Aos profissionais da área e estudantes o espaço será uma oportunidade de atualização relacionada à produção de hortaliças e ao público em geral uma forma de conhecer o trabalho desenvolvido na universidade e obter conhecimento sobre a pesquisa e produção de hortaliças.
The objective of the present study was to assess the influence of the packaging material (cotton cloth bag, multifolium paper and glass container) and storage environment (laboratory and cold chamber conditions) on fennel seedling growth. Seeds were placed in different packaging materials and exposed to the two environments for twelve months. Assessments were made before storage and at every two months and the following were determined: total emergence, emergence speed index, seedling fresh and dry matter. A completely randomized experimental design was used, with four replications in a 2 x 3 x 6+1 factorial design in split split plots, where the plot was the storage environment (laboratory and cold chamber conditions); the split plot was the storage (cotton cloth bag, multifolium paper and glass container) and the split plot was the storage periods (2, 4, 6, 8, 10 and 12 months) and plus an additional treatment (before storage). The results showed that the fennel seedlings had superior growth when their seeds were placed in the glass packaging material, regardless of the storage environment.
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