Alfalfa (Medicago sativa L.) is a major cultivated forage legume crop, used mainly as forage and its benefits in sustainable cropping system in agriculture. 'Tomin' is an alfalfa native variety cultivated in large scale due to its superior characteristics and adaptability to dry conditions. Ecotypes of Tomin were grown in different regions, they show variation on their morphological and agronomical characteristics, and therefore knowledge on the level of the genetic diversity among these ecotypes would assist in introducing this crop in breeding programs to produce high quality and drought resistant genotypes. The aim of current study was to molecularly characterize four ecotypes belonging to alfalfa 'Tomin' variety cultivated into two different ecoclimatic regions. The AFLP fingerprinting using two EcoRI/ MseI enzyme combination and twelve selective primer pairs was carried out to elucidate the genetic diversity among alfalfa ecotypes. The results revealed a high variation among the alfalfa ecotypes analysed, they showed the mean similarity of 38% among them. The UPGMA based cluster analysis and Principal Coordinate Analysis (PCoA) showed that alfalfa ecotypes cultivated in two different geographical areas did not cluster according to their geographical origin, however higher similarity was observed between ecotypes from Fushe-Kruje region than between the ecotypes of Gjirokaster.
Ö ZF LT3 akut miyeloid lösemide farklı genetik mutasyonlara duyarlı tip 3 tirozin kinaz reseptörüdür. Birçok çalışma, çok sayıda hastada (yaklaşık üçte biri) bu genin mutasyona uğradığını göstermiştir; bunun nedeni aşağı akış sinyal yolağı ve anormal hücre çoğalmasının yol açtığı iç tandem duplikasyonlardır (ITD). Bu çalışmada, NOX4 inhibisyonunun kombine etkilerini ve FLT3-ITD sinyal iletiminde FLT3-ITD tirozin kinazın inhibisyonunu aydınlatmak için özgül hedef inhibitörleri kullanılmıştır. Anahtar KelimelerFLT3, tirozin kinaz, inhibitör. A B S T R A C T FLT3 is a type 3 tyrosine kinase receptor susceptible to different genetic mutations in acute myeloid leukemia. Several studies have shown that a large number of patients (approximately one third) have mutations of this gene, lead of which is internal tandem duplications (ITD) that leads to activation of downstream signaling pathway and abnormal cellular growth. We used specific target inhibitors to elucidate the combined effects of NOX4 inhibition and the inhibition of FLT3-ITD tyrosine kinase on FLT3-ITD signal transduction.
AML (acute myeloid leukemia) is an aggressive hematopoietic malignancy with multiple signaling pathways contributing to its pathogenesis. A key role in of these pathways is the FLT3 (FMS-like tyrosine kinase receptor-3). Activation of the FLT3ITD (internal tandem duplication of FLT3) leads to decreased progression and low survivability rate. Targeting the kinase activity of FLT3 with inhibitory compounds can be used as an obvious therapeutic option. The second generation inhibitors have shown enhanced FLT3 specificity and good results in targeting AML. The aim of this study is to elucidate the combined effects of these inhibitors on FLT3ITD signal transduction.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.