A confirmatory multi-class LC-MS/MS method have been developed for simultaneous determination of 23 antibiotic drugs from seven different classes in bovine milk. The method was validated in accordance with the criteria prescribed in Commission Decision 2002/657/EC. The linear regression analysis showed good correlation with R2 > 0.9800. LOD are in the range of 0.17-6.94 ng/ml, while the LOQ are in the range of 0.50-22.71 ng/ml. The CCα range from 4.43 to 122.33 ng/mL and CCβ was from 4.88 to 139.78 ng/mL. The recovery of the method ranged from 71.96 to 108.70%. The coefficient of variation for repeatability varied from 1.08 to 20.28% and the coefficient of reproducibility varied from 3.14 to 22.88%. In the present study, 189 bovine milk samples were collected from dairy farms and analyzed using confirmatory multi-class LC-MS/MS method. A total of 14 (7.41%) samples were found positive for antibiotics and sulfonamides. The concentrations of the residues were below the maximum levels established by EU.
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