This study aimed to determine bio-ethanol potential of poultry manure. The manure was hydrolyzed by dilute H 2 SO 4 (0.2, 0.4, 0.6, 0.8 and 1 M) at temperature of 100 and 130°C for 30 min. Maximum glucose (115.6 g/l) was obtained from hydrolysis of 100 g poultry manure by 0.8 M H 2 SO 4 at 100°C for 30 min. The study shows that treatment of poultry manure releases more total reducing sugar using 0.8 M H 2 SO 4. Refractometry analysis of the fermented hydrolysate gave maximum ethanol concentration of 5% w/v with 0.8 M H 2 SO 4 and 4, 4, 3 and 2.5% of ethanol with 1, 0.6, 0.4 and 0.2 M H 2 SO 4 hydrolysis at 130°C for 30 min, respectively. Treatment with 0.8 M acid is an effective way that results to maximum yield and treatment with 0.2 M acid is the least effective. The optimum fermentation time is 24 h.
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