The Blade Tip Timing (BTT) measurement system is a technique to measure vibration parameters of a rotating bladed disk. In particular for synchronous vibrations the BTT provides signals versus the rotation speed of the disk starting from the measurement of the time of arrival (TOA) of each blade under the tip timing probes. The signals must be post processed in order to obtain the interesting parameters of each blade vibration. The paper presents a method to extract the main parameters (amplitude and frequency) in resonance condition from the tip timing measurements. The proposed method is a revision of the already existing well known Two-Parameter Plot (2PP) method which requires a minimum of two probes. Improvements to the existing 2PP method are here suggested mainly in the part of engine order identification. The proposed method is then applied to the BTT measured signals coming from a rotating bladed disk excited at different engine orders. At the same time on the disk the vibration of one blade was detected by strain gauges. The strain gauges were calibrated and they provide the reference values of the vibration parameters. The vibration parameters derived by the proposed method are in agreement with those obtained by the strain gages methodology.
Most aircraft turbojet engines consist of multiple stages coupled by means of bolted flange joints which potentially represent source of nonlinearities due to friction phenomena. Methods aimed at predicting the forced response of multistage bladed disks have to take into account such nonlinear behavior and its effect in damping blades vibration. In this paper, a novel reduced order model (ROM) is proposed for studying nonlinear vibration due to contacts in multistage bladed disks. The methodology exploits the shape of the single-stage normal modes at the interstage boundary being mathematically described by spatial Fourier coefficients. Most of the Fourier coefficients represent the dominant kinematics in terms of the well-known nodal diameters (standard harmonics), while the others, which are detectable at the interstage boundary, correspond to new spatial small wavelength phenomena named as extra harmonics. The number of Fourier coefficients describing the displacement field at the interstage boundary only depends on the specific engine order (EO) excitation acting on the multistage system. This reduced set of coefficients allows the reconstruction of the physical relative displacement field at the interface between stages and, under the hypothesis of the single harmonic balance method (SHBM), the evaluation of the contact forces by employing the classic Jenkins contact element. The methodology is here applied to a simple multistage bladed disk and its performance is tested using as a benchmark the Craig–Bampton ROMs of each single stage.
Voltage-Dependent Anion-selective Channel isoform 1 (VDAC1) is the most abundant isoform of the outer mitochondrial membrane (OMM) porins and the principal gate for ions and metabolites to and from the organelle. VDAC1 is also involved in a number of additional functions, such as the regulation of apoptosis. Although the protein is not directly involved in mitochondrial respiration, its deletion in yeast triggers a complete rewiring of the whole cell metabolism, with the inactivation of the main mitochondrial functions. In this work, we analyzed in detail the impact of VDAC1 knockout on mitochondrial respiration in the near-haploid human cell line HAP1. Results indicate that, despite the presence of other VDAC isoforms in the cell, the inactivation of VDAC1 correlates with a dramatic impairment in oxygen consumption and a re-organization of the relative contributions of the electron transport chain (ETC) enzymes. Precisely, in VDAC1 knockout HAP1 cells, the complex I-linked respiration (N-pathway) is increased by drawing resources from respiratory reserves. Overall, the data reported here strengthen the key role of VDAC1 as a general regulator of mitochondrial metabolism.
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