This study was carried to identify the causes of pancytopenia and to fi nd out the bone marrowmorphology in cases of pancytopenia.It was a cross sectional study conducted over a period of two years in the Department of Pathology,Tribhuvan University Teaching Hospital. Bone marrow aspiration smears of patients fulfi lling thecriteria of pancytopenia were examined. The data obtained were analyzed using measures of centraltendency.One hundred and forty eight cases underwent bone marrow aspiration and it constituted 15.74% oftotal cases. Mean age was 30 years (range, 1-79 years). 42 cases were children (28.37%). Male: femaleratio was 1.5:1. The commonest cause was hypoplastic bone marrow seen in 43 cases (29%) followedby megaloblastic anemia in 35 cases (23.64%), and hematological malignancy in 32 cases (21.62%).Erythroid hyperplasia was seen in 29 cases (19.6%) and normal bone marrow was seen in 5 cases(3.38%). There was one case each of Niemann-Pick disease and metastatic neuroblastoma in childrenand chronic pure red cell aplasia and leishmaniasis in adults. Acute leukemia was the commonesthematological malignancy. In children, commonest fi nding was hypoplastic bone marrow (38.1%)while in adults megaloblastic anemia (30.18%) was commonest fi nding followed by hypoplasticanemia (25.47%).In present study bone marrow examination was able to establish diagnosis in 77% of cases. Hypoplasticmarrow was the commonest diagnosis, followed by megaloblastic anemia, and hematologicalmalignancies.Key Words: Bone marrow aspiration, hypoplastic anemia, leukemia, megaloblastic anemia,pancytopenia

Background: In leprosy, there is a range of varied clinicopathologic manifestations and the diagnosis is made from adequate clinical information combined with bacilloscopy and histopathology which helps in diagnosing different types of leprosy and separating it from other granulomatous lesions. Aim of the study was to classify leprosy according to Ridley Jopling classification and perform the clinicopathological correlation. Materials and Methods: A cross sectional comparative study of skin biopsies of newly diagnosed leprosy recieved over a period of 18 months from January 2009 to June 2010 and clinicopathologic correlation was done along with special stain. Results: This study included 75 patients diagnosed clinically as leprosy. Skin biopsy revealed evidence of leprosy in 72 cases. Maximum number of patient clinically belonged to tuberculoid leprosy which constituted 25 (33%) cases followed by borderline tuberculoid 19 (25.33%). On the contrary, histologically borderline tuberculoid was the most common type (40%, n=30) cases and tuberculoid leprosy constituted (13.33%, n=10) cases. Three cases of clinically diagnosed tuberculoid leprosy showed no features of leprosy histologically. Clinical and histopathological correlation was seen in 34 cases (45.33%). The correlation was highest in borderline tuberculoid (63.15%) followed by borderline lepromatous and lepromatous leprosy. Slit skin smear was positive in 31 cases (43.05%). Fite Farraco stain was positive in 18 cases (25%). Conclusion: The classification of leprosy requires attention to the histopathological criteria and correlation with clinicalinformationand bacteriological examination so as to facilitate accurate therapy to prevent undesirable complication. DOI: http://dx.doi.org/10.3126/jpn.v3i6.8992   Journal of Pathology of Nepal (2013) Vol. 3, 452-458

Background: Pancytopenia is a common hematological finding resulting from varieties of disease processes that require evaluation of bone marrow. This study was carried out to evaluate bone marrow findings in patients presenting with pancytopenia. Materials and Method:This was a prospective cross sectional study carried out to identify the causes of pancytopenia based on bone marrow examination. Bone marrow examinations were performed in 503 cases for different indications over a period of one year. Results:One hundred and two (20.27%) cases fulfilled the criteria of pancytopenia. Trephine biopsy was possible only in 48 cases. In 75% cases aspiration findings were similar to biopsy. Mean age of patients was 38.8 years. Maximum number of cases was seen in age group of 15-30 years. Hypoplastic anemia was the commonest cause followed by hematological malignancies, megaloblastic anemia, leishmaniasis and Gaucher disease. Bone marrow examination alone was able to establish the diagnosis in 76.5% cases. In rest marrow findings were nonspecific and in 4.9% cases findings were normal. Conclusion:Bone marrow aspiration coupled with trephine biopsy can diagnose majority but not all the cases of pancytopenia. Hypoplastic anemia, hematological malignancies and megaloblastic anemia are the commonest causes of pancytopenia. Maximum diagnostic yield can be achieved by correlation with clinical findings, peripheral blood findings and with other laboratory and radiological parameters.

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Background Renal tumor is the 13th most common malignancy in the world and more than 90% of renal tumors are renal cell carcinomas. As there is no data available on renal cell carcinoma in Nepal, hence this study was undertaken to analyze the patterns of renal cell carcinoma in patients with renal mass at a tertiary level hospital in Nepal. Objectives To analyze the patterns of renal cell carcinoma in patients with renal mass at a tertiary level hospital in Nepal. Methods The case records of 50 consecutive patients with renal cell carcinoma presenting at the Tribhuvan University Teaching Hospital, Kathmandu from July 2006 to June 2011 were retrospectively evaluated for presenting symptoms, physical finding, investigation and histopathology report. Results Out of 50 patients, 64% were male and 36% were female. The age ranged between 11 to 78 years (mean ± SD: 55 ± 15 years). Fifty four percent of patients were smokers. Incidentally tumor was detected in 40% cases by ultrasonography and the typical triad was present in only 4%. The tumor was occupying upper pole in 40% of cases. The tumor size ranged from 3 to 15 cm (mean ± SD: 7.3 ± 2.9 cm). Histopathologically, 76% of the patient had organ confined renal cell carcinoma (T1-2 N0 M0). Clear cell was the most common type seen in 86%. Fuhrman’s nuclear grade 2 was found in 50%. ConclusionMany of the renal cell carcinoma are detected incidentally, at an early stage and are of clear cell subtype.DOI: http://dx.doi.org/10.3126/kumj.v9i3.6302 Kathmandu Univ Med J 2011;9(3):185-8

Retrospective analysis of 321 cases of breast cancer diagnosed in T. U Teaching Hospitalin a period of 10 years, from May, 1991 to April, 2000 was carried out. There were317 cases (98.8%) females and 4 cases males (1.2%). The youngest patient was 22year old female and oldest patient was 92 year old female. The most common agegroup according to frequency was in forties (34.6%) followed by in thirties (25.5 %).Mean duration of symptoms before coming to doctor was 8.3 months and mean size oftumor was 6 cm. Out of 317 females, 310(97.2%) were married and average numberof children was 3. Out of 289 cases diagnosed as malignancy with fine needle aspirationcytology (FNAC) 279 (96.5%) was also diagnosed as malignancy in biopsy and theremaining 10 cases as non malignant diseases were diagnosed as malignancy in biopsywith a false negative rate of 3.5%. The histological types of breast cancer cases wereInfiltrating Ductal Carcinoma 280 cases (87.2%), Medullary Carcinoma 11 cases(3.4%), Infiltrating Lobular Carcinoma 5 cases (1.6%), Mucinous Carcinoma 4 cases(1.2%), Sarcoma 4 cases (1.2%), Squamous Cell Carcinoma 4 cases (1.2%), PapillaryCarcinoma 3 cases (0.9%), Tubular Carcinoma 2 cases, Adenosquamous Carcinoma2 cases (0.6%), Intraductal Carcinoma 2 cases (0.6%) and Non-Hodgkins Lymphoma1 case. Out of 305 operated cases, the types of operation performed was ModifiedRadical Mastectomy in 208 cases (68 %) and palliative mastectomy in 72 cases (23.5%),only lumpectomy in 24 cases (7.9%) and others in 2 cases. Among 246 cases withavailable axillary lymph node biopsy, there was metastatic diseases in 146 cases (60%)of cases. The Breast cancer was diagnosed in advanced stages in 63% of cases. StageIIIA (24%), Stage IIIB (21.5%) and Stage IV (17.4%). Breast cancers were diagnosedin advanced stages in below 40 age group in 65 out of 90 cases (72.2 % ) comparedto137 out of 231 cases ( 59.3 % ) in above 40 age group.Key Words: Breast cancer, Advanced stage, FNAC, Surgery.

Fine needle aspiration cytology of superficial as well as of deep seated lesions is now well recognized in diagnosis of neoplastic as well as nonneoplastic and inflammatory lesions. Recently it has also gained popularity for its diagnostic and therapeutic role in male infertility. The purpose of this article was to review various studies published on role of testicular fine needle testicular cytology in male infertility and provide a brief information on method of testicular fine needle aspiration, interpretation of testicular fine needle aspiration cytology for evaluation of spermatogenesis, its advantages, limitations and complications as compared to testicular biopsy.

Background: Granulomatous inflammation is a special type of chronic inflammation that is being a manifestation of many infective, toxic, allergic, autoimmune and neoplastic diseases and also conditions of unknown etiology. Materials and Methods: The study included a total of 418 granulomatous lesions, diagnosed from January 2010 to December 2012 in the department of pathology, Tribhuvan University Teaching Hospital, Kathmandu, Nepal. Results: The median age of the patients with granulomatous lesion was 29 years and the majority of the patients were in the age group of 20-29 yrs with slight male predominance. Majority of granulomas were seen in lymph nodes (41.1%), followed by skin & subcutis (22%), and bone & joints (11.5%). Tuberculosis was the most common cause of granuloma with 258 (61.7%) cases, followed by fungal infections, foreign body reaction, parasites and toxoplasmosis. Out of 258 cases of tuberculosis, lymph nodes were involved in 140 (54.26%) cases, followed by bone & joints (12.79%) and respiratory system (5.42%). The most common type of granuloma was epithelioid (83.5%), followed by epithelioid with suppuration and mixed inflammatory. Conclusion: The granulomatous lesion is common in third decade of life with slight male predominance. The commonest site is lymph node with tuberculosis being the most common cause and epithelioid being the most common type of granuloma. DOI: http://dx.doi.org/10.3126/jpn.v3i6.8994   Journal of Pathology of Nepal (2013) Vol. 3, 464-468