The aim of this in vitro study was to evaluate the early cell response and protein adsorption elicited by the argon plasma treatment of different commercially available titanium surfaces via a chair-side device. Sterile disks made of grade 4 titanium (n= 450, 4-mm diameter) with 3 surface topographies (machined, plasma sprayed, and zirconia blasted and acid etched) were allocated to receive 4 testing treatments (2% and 10% protein adsorption and cell adhesion with MC3T3-E1 and MG-63). Furthermore, the specimens were divided to undergo 1) argon plasma treatment (10 W, 1 bar for 12 min) in a plasma reactor, 2) ultraviolet (UV) light treatment for 2 h (positive control group), or 3) no treatment (control group). Pretreatment surface analyses based on a scanning electron microscope and profilometer images were also performed. Profilometric analysis demonstrated that the evaluated specimens perfectly suit the standard parameters. The use of argon plasma was capable of affecting the quantity of proteins adsorbed on the different surfaces, notwithstanding their roughness or topographic features at a low fetal bovine serum concentration (2%). UV light treatment for 2 h attained similar results. Moreover, both the plasma of argon and the UV light demonstrated a significant increase in the number of osteoblasts adherent at 10 min in all tested surfaces. Within its limitations, this in vitro study highlights the potential biological benefits of treating implant surfaces with plasma of argon or UV, irrespective of the roughness of the titanium surface. However, in vivo experiments are needed to confirm these preliminary data and settle the rationale of a treatment that might be clinically relevant in case of bone-reparative deficiencies.
Silver nanocluster–silica matrix composite coatings have been deposited by radio frequency (RF) co‐sputtering on silica substrates. Field emission scanning electron microscopy and X‐ray diffraction spectra of the as deposited and heated samples (150–600 °C) revealed the presence of metal silver nanoclusters, their size depending on the heating treatment. The antibacterial activity of the as deposited and heated samples has been measured in accordance to National Committee for Clinical Laboratory Standards, and it has been demonstrated on samples heated up to 450 °C in contact mode and for samples heated at 600 °C in a liquid environment. Their antibacterial activity was still present after gamma ray and ethylene oxide gas (EtO) sterilization of the samples. Silver leaching tests on the as deposited and heated samples has been measured by graphite furnace atomic absorption spectrometer, revealing an amount ranging from 0.1 to 0.9 µg mm−2, over 28 days. Tape resistance (ASTM D3359‐97) and scratch resistance tests have been done on each sample revealing a good adhesion of the coatings on silica.
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