Kasus anemia di Indonesia masih cukup tinggi hasil menunjukkan bahwa angka prevalensi anemia secara nasional pada semua kelompok umur adalah 21,70 %. Sedangkan prevalensi anemia di Provinsi Jawa Timur sebesar 5,8 %. Anemia adalah kondisi penyakit dimana jumlah sel darah merah lebih rendah dari jumlah normal. Kondisi ini biasa disebut sebagai kekurangan darah. Anemia dapat dideteksi dengan melakukan pengukuran kadar hemoglobin (Hb) menggunakan metode POCT (Point of Care Testing). Metode POCT merupakan metode yang dilakukan untuk pemeriksaan sederhana dengan menggunakan sampel dalam jumlah sedikit, mudah, cepat serta efektif dilakukan di daerah-daerah dengan jumlah fasilitas kesehatan seperti pusat kesehatan masyarakat (puskesmas), rumah sakit yang relatif sedikit khususnya di lokasi pengabdian masyarakat di Desa Sumbersono, Mojokerto. Jenis metode yang digunakan adalah observasional analitik. Pengabdian masyarakat ini bertujuan untuk skrining (pemeriksaan) kadar hemoglobin sebagai deteksi dini penyakit anemia dengan metode POCT, mengetahui hubungan antara umur, pendidikan, pekerjaan terhadap kejadian anemia serta sebagai upaya preventif (pencegahan) untuk meminimalisir penyakit anemia. Hasil menunjukkan dari 48 reponden (laki-laki 11 orang), (perempuan 37 orang) bahwa kejadian anemia pada perempuan 40 % lebih besar dibandingkan dengan laki-laki 6 %. Jumlah kasus anemia terbanyak terjadi pada umur ≥ 60 presentase 36,36 % dibandingkan kelompok umur lainnya. Jumlah kejadian anemia terbanyak dengan presentase 46 % pekerjaan Petani dibandingkan pekerjaan lainnya. Jumlah anemia dengan pendidikan sekolah dasar presentase 16,67 % terbanyak dibandingkan dengan pendidikan lainnya. Kesimpulannya tidak terdapat hubungan antara umur, pendidikan, pekerjaan terhadap penyakit anemia bagi masyarakat desa Sumbersono, Mojokerto.Kata kunci: Anemia, Kadar Hemoglobin . POCT, Faktor AnemiaAbstract Cases of anemia in Indonesia are still quite high. The results show that the national prevalence of anemia in all age group is 21.70%. While the prevalence of anemia in East Java Province was 5.8%. Anemia is a disease because the number of red blood cells is lower than the normal number. This condition is commonly referred to blood deficiency. Anemia can be detected by measuring of hemoglobin (Hb) levels using the POCT (Point of Care Testing) method. The POCT method is a method that is carried out for a simple examination using a small samples, easy, fast and effective carried out in areas with a number of health facilities such as community health centers, hospitals that are relatively small, especially in community service locations in the village Sumbersono, Mojokerto. The type of method used is analytic observational. This community service activities aims to screening (examining) hemoglobin levels as an early detection of anemia using the POCT method, to find out the relationship between age, education, work and anemic events and as a preventive measure (prevention) to minimize anemia. The results showed there were 48 population, while (11 men), (37 women) that the incidence of anemia in women was 40% greater than 6% men. The highest number of anemia cases occurred at age ≥ 60 is 36.36% compared to other age groups. The highest number of anemia occurrences with 46% of Farmers' work compared to other occupations. The highest number of anemia with primary school education level is 16.67% compared to other education levels. Conclution There was no significant correlation between age, educational background, works and anemia cases in community the Sumbersono village, Mojokerto.
Pengambilan sampel, khususnya spesimen darah, pada penelitian bidang medis memiliki tantangan tersendiri. Ini dikarenakan darah merupakan spesimen yang paling mudah mengalami perubahan jika sudah dikeluarkan dari tubuh, baik akibat faktor fisiologis maupun lingkungan. Oleh karena itu, jika ditangani dengan tidak tepat, sampel tersebut dapat memberikan informasi klinis yang berbeda. Buku di tangan Anda ini merupakan satu upaya untuk menanggulangi persoalan tersebut sebab buku ini berisi pengetahuan tentang berbagai teknik pengambilan darah vena, transportasi hingga pengolahan spesimen. Dalam buku ini juga terdapat informasi tentang wawasan dasar, peralatan, dan penjaminan mutu pada pengambilan spesimen darah manusia. Itulah sebab, buku ini diharapkan dapat menjadi acuan bagi para peneliti, kalangan tenaga kesehatan dan akademis, atau siapa saja yang hendak melakukan pengambilan darah vena sebagai sampel pada penelitian.
Many blood collection tube manufacturers do not recommend shaking the red tube. Shaking the red tube to avoid the intensity of the changed of blood that interacts with the glass surface of the tube will trigger the contact path in the coagulation cascade. Generally, the blood takes 30-60 minutes to clots at room temperature without shaking. The aim of this study was to determine the effectiveness of shaking the red-cap blood collection tube in producing serum volume. The method of this study was experimental As much as 5 mL of blood was taken and put into 3 tubes with a volume of 1mL each tube. The first and the second tube were shaken 8 times. The first tube was incubated for 10 minutes while the second tube was incubated for 25 minutes. Meanwhile, the third tube (as a control) was not shaken but was incubated for 40 minutes. The tube were centrifuged at 3000 g for 10 minutes. The serum volume was measured using micropipette and collected into Eppendorf tube. The results showed that there were a difference in the number of serums formed after tube shaking by time variation (P = 0.002), the results of the Post Hoc Test using Bonferroni test while showed that the second tube did not have a difference in serum volume with control (P = 0.751). It can be concluded that the red-cap blood collection tube, which was shaken 8 times and incubated for 25 minutes long could accelerate the coagulation process.
<p>Nilai normal Hb ditentukan dari kadar Hb itu sendiri. Kadar Hb ibu hamil terjadi jika produksi sel darah merah meningkat, nilai normal hemoglobin (12 sampai 16 gr/dl) dan nilai normal hematokrit (37% sampai 47%) menurun secara mencolok. Anemia dalam kehamilan adalah kondisi ibu dengan kadar hemoglobin (Hb) < 11 gr% pada trimester I dan III sedangkan pada trimester II kadar hemoglobin < 10,5 gr%.</p><p>Penelitian ini bertujuan untuk mengetahui hubungan antara karakteristik dan kadar Hb ibu hamil trimester III. Penelitian dilakukan pada bulan Maret-Mei 2019 di Puskesmas Jagir Surabaya. Metode penelitian yang digunakan adalah cross sectional dan pengambilan sampel dengan simple random sampling sebanyak 30 responden ibu hamil trimester III yang berkunjung.</p><p>Hasil penelitian menunjukkan bahwa lebih dari setengah (56,7%) memiliki kadar Hb>11g% dan hampir setengah (43,3% responden yang memiliki kadar Hb <span style="text-decoration: underline;"><</span>11g%. Hasil analisis bivariat menggunakan chi square dengan p value <0,05 mendapatkan hasil adanya hubungan antara usia dan kadar Hb ibu hamil trimester III yaitu 0,001 , terdapat hubungan antara paritas dan kadar Hb ibu hamil yaitu 0,003 , tidak terdapat hubungan antara pendidikan dan kadar Hb ibu hamil trimester III yaitu 0,11 serta tidak ada hubungan antara pekerjaan dan kadar Hb ibu hamil trimester III yaitu 0,72.</p><p>Disarankan kepada petugas untuk memberikan pendidikan kesehatan pada ibu hamil untuk meningkatkan pengetahuan ibu hamil tentang bahaya anemia dalam kehamilan dan pentingnya mengkonsumsi tablet fe dan makanan yang mengandung zat besi.</p>
Complete blood count (CBC) is one of the laboratory tests most often influenced by doctors. The use of a hematology analyzer offers a wider range of probe parameters. The pre-analytic stage accounts for 70% of errors, one of which is the delay of the examination. Changes in report results were reported due to changes in blood cell morphology due to EDTA additives and room temperature. The aim of this research is the disturbance of stability of the results of examination of various CBC parameters in blood samples that are left at room temperature for 24 hours using a hematology analyzer. This experimental laboratory research was conducted at the Pramita Jemur Andayani Clinical Laboratory. Blood samples were obtained from volunteers, stored at room temperature and subjected to immediate examination (control) and after a delay of 6, 12 and 24 hours (treatment). A total of 30 respondents, consisting of 8 men and 22 women. The mean age of the respondents was 22 ± 1 year. There was no difference in the results of the examination (p-value > 0.05) in the RBC, Hct MCV, MCHC, PLT and PDW. The results of the examination (p-value < 0.05) were found on Hgb, MCH, RDW, WBC, NEU, IG, MONO, EO, BASO, LYM, PLT and PDW. Delayed CBC examinations using the CELL-DYN Ruby hematology analyzer directly gave different results on several parameters ranging from 6 hours delay of examination.
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