초 록 본 연구에서는 항산화성분 함량이 높다고 알려진 천연물인 감잎, 엉겅퀴, 뉴그린을 이용하여 유효성분을 추출하고 DPPH radical scavening activity와 플라보노이드성분 함량분석을 수행하였다. 추출에 사용된 추출용매로는 초순수와 주정을 이용하였고, 초순수-주정의 비율, 시료의 양, 추출시간, 추출온도 등을 변수로 설정하였다. 각 천연물의 최적추 출조건은 추출시간(2.5~3.5 h), 시료의 양은 50 g/L이었고, 주정/초순수의 부피비와 추출온도의 경우 각각 감잎(55~65 vol%, 50~60 ℃), 엉겅퀴(40~50 vol%, 55~65 ℃), 뉴그린(55~65 vol%, 50~60 ℃)으로 나타났다. 또한 추출된 유효성분 의 항산화능과 플라보노이드성분 함량분석 결과 감잎 > 엉겅퀴 > 뉴그린 순으로 크게 나타났다. AbstractIn this study, we extracted active components from thistle, persimmon leaf, and new green which are known to have a high content of antioxidants and also analyzed the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavening activity and flavonoid content. Both ultrapure water and alcohol were used as extraction solvents and the ratio of both solvents, sample, amunts extraction time, and extraction temperature were varied. The optimal extraction condition of each natural compounds were 2.5~3.5 h of the extraction time and 50 g/L of the sample amount. The optimal ratio of ultrapure water and alcohol and extraction temperature were as follows; persimmon leaf (55~65 vol%, 50~60 ℃), thistle (40~50 vol%, 55~65 ℃) and new green (55~65 vol%, 50~60 ℃). In addition, the antioxidant capacity and flavonoid content of the extract increased in the order of persimmon leaf, thistle, and new green. Keywords
Phytoestrogens are one of quasi-estrogens which are not generated within the endocrine system, but consumed by intaking phytoestrogenic plants. Phytoestrogens, also called as "dietary estrogens", are various botanic chemical compound groups naturally occurring nonsteroidal plant compounds of spontaneous generation. Due to their structural similarities to estrogone, they may provide desirable health benefits for reducing the menopausal symptoms. In this paper, various phytoestrogens were extracted from sophora root, pomegranate and kudzu by ultrasonic extraction process. In this study, the amount of extracted phytoestrogens at various ultrasonic power strengths and extraction times were compared, which could be used as basic data in the study of biology and chemistry related to natural products.
In this study, the extractable content of each parameters from angelica was confirmed using conventional solvent extraction and microwave extraction in order to extract the functional active ingredient. In addition, the functionality of active ingredients was evaluated by measuring the antioxidant activity and the flavonoids and total polyphenols of the active ingredient extracted from angelica, For the conventional solvent extraction at optimal conditions of the extraction time (2 h), ethanol/pure water volume ratio (60%), the extraction temperature (60 ℃), 20.6 wt% of the active ingredient were extracted. Also, when using microwave extraction at optimal conditions of the microwave irradiation time (6 min), microwave intensity (600 W) and ethanol/pure water volume ratio (60 vol%) 22.8 wt% of the active ingredient were extracted. The microwave method required shorter time to complete extraction compared to that of using the conventional solvent extraction method. The antioxidant activity of active ingredients extracted from angelica was 31.46% of DPPH radical scavening activity. The flavonoid content was 14.20 mg QE/mg dw, and total polyphenol content was 11.70 mg GAE/g when using the microwave extraction process.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.