Bacterial resistance to antibiotics has become a public health issue. Over the years, pathogenic organisms with resistance traits have been studied due to the threat they pose to human well-being. However, several studies raised awareness to the often disregarded importance of environmental bacteria as sources of resistance mechanisms. In this work, we analyze the diversity of antibiotic-resistant bacteria occurring in aquatic environments of the state of Rio de Janeiro, Brazil, that are subjected to distinct degrees of anthropogenic impacts. We access the diversity of aquatic bacteria capable of growing in increasing ampicillin concentrations through 16S rRNA gene libraries. This analysis is complemented by the characterization of antibiotic resistance profiles of isolates obtained from urban aquatic environments. We detect communities capable of tolerating antibiotic concentrations up to 600 times higher than the clinical levels. Among the resistant organisms are included potentially pathogenic species, some of them classified as multiresistant. Our results extend the knowledge of the diversity of antibiotic resistance among environmental microorganisms and provide evidence that the diversity of drug-resistant bacteria in aquatic habitats can be influenced by pollution.
Despite a great number of published studies addressing estuarine, freshwater and marine bacterial diversity, few have examined urban coastal lagoons in tropical habitats. There is an increasing interest in monitoring opportunistic pathogens as well as indigenous microbial community members in these water bodies by current molecular and microbiological approaches. In this work, bacterial isolates were obtained through selective plate dilution methods to evaluate antibiotic resistances. In addition, 16S rRNA gene libraries were prepared from environmental waters and mixed cultures grown in BHI medium inoculated with Jacarepaguá lagoon waters. Denaturing gradient gel electrophoresis (DGGE) analyses showed distinct community profiles between environmental communities from each studied site and their cultured counterparts. A total of 497 bacterial sequences were analyzed by MOTHUR, yielding 245 operational taxonomic units (OTUs) grouped at 97% similarity. CCA diagrams showcased how several environmental variables affect the distribution of 18 bacterial orders throughout the three distinct habitats. UniFrac metrics and Venn diagrams revealed that bacterial communities retrieved through each experimental approach were significantly different and that only one OTU, closely related to Vibrio cholerae, was shared between them. Potentially pathogenic bacteria were isolated from most sampled environments, fifty percent of which showed antibiotic resistance.
O presente estudo teve os seguintes objetivos propostos: 1)Identificar a prevalência de indivíduos adultos ativos fisicamente com dores nas costas; 2) Identificar a prevalência de dores nas costas em cada modalidade esportiva praticada por adultos ativos fisicamente. Foram utilizadas as informações dos indivíduos selecionados para responder o questionário individual da Pesquisa Nacional de Saúde (PNS). A amostra do presente estudo foi composta 15043 indivíduos, com faixa de idade entre 18 e 59 anos de idade, praticantes de atividade física, que responderam todas as questões selecionadas. A análise estatística dos dados foi realizada de maneira descritiva, para análise da prevalência de indivíduos adultos ativos fisicamente com dores nas costas e, ainda, entre os praticantes de cada modalidade de atividade física relatada. Nos resultados foram apresentados valores de 2055 (15,9%) indivíduos adultos ativos fisicamente com dores nas costas. A prevalência em mulheres (19,6%) e em adultos de meia idade (24,2%) tende a ser maior que em homens (12,0%) e em adultos mais jovens (12,6%), respectivamente. A caminhada foi a atividade mais procurada e a prevalência entre praticantes foi de 20,3%. Outras atividades que podem trazer benefícios às dores nas costas apresentaram prevalências consideradas altas. Por modalidades específicas de atividade física, os valores de prevalência de dores nas costas são variados quando a população é dividida em grupos por sexo e idade. Os valores de prevalência encontrados devem ser considerados preocupantes, principalmente no que tange à indicação de uma atividade física adequada e à prescrição de exercícios com o devido controle da intensidade.
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