The gene coding for the phaseolotoxin-insensitive ornithine carbamoyltransferase (OCTase) from Pseudomonas syringae pv. phaseolicola has been cloned and sequenced. The gene has a deduced coding capacity for a polypeptide with a calculated Mr of 36,520 daltons. Comparison of the amino acid sequence of the OCTase enzymes encoded by the P. aeruginosa argF and the Escherichia coli argI and argF genes with the deduced sequence of the newly identified gene shows that 79 amino acid residues are strictly conserved in all four polypeptides; among these 7 out of 9 residues are involved in enzyme function. Of three amino acid regions that have been implicated in substrate binding or catalysis, two are strictly conserved, and the third involved in carbamoylphosphate binding differs. This correlates well with published data showing that phaseolotoxin competes for the carbamoylphosphate binding site in the phaseolotoxin-sensitive OCTases. We propose that the gene be named argK.
As compared to AC microgrids, DC microgrids reduce the hardware complexity of converter-dominated power distribution in the presence of a high number of renewable energy sources, energy storage systems and energy efficient loads. Another frequently highlighted advantage is their resiliency and tolerance against AC grid disturbances resulting in improved overall power quality. However, with respect to power quality, the question arises whether existing international power quality standards and metrics remain applicable in DC microgrids or require adjustments. Therefore, this paper critically revises the definitions and power quality indicators specified in IEC 61000 and IEEE Std1159. The resulting review is essential to unambiguously define the responsibilities of the microgrid operators, customers and device manufacturers. Apart from that, causes and consequences of power quality issues in DC microgrids are discussed.
This paper will revise, experimentally investigate, and discuss the main application challenges related to gallium nitride power semiconductors in switch-mode power converters. Gallium Nitride (GaN) devices are inherently gaining space in the market. Due to its high switching speed and operational switching frequency, challenges related to the circuit design procedure, passive component selection, thermal management, and experimental testing are currently faced by power electronics engineers. Therefore, the focus of this paper is on low-voltage (<650 V) devices that are used to assemble DC-DC and/or DC-AC converters to, for instance, interconnect PV generation systems in the DC and/or AC grids. The current subjects will be discussed herein: GaN device structure, the advantages and disadvantages of each lateral gallium nitride technology available, design challenges related to electrical layout and thermal management, overvoltages and its implications in the driver signal, and finally, a comprehensive comparison between GaN and Si technology considering the main parameters to increase the converters efficiency.
Low Voltage DC microgrids emerge as a viable alternative to AC microgrids. A large research interest is noted towards fast and selective protection of DC grids, typically focusing on hybrid or full solid state solutions. In this paper, the use of fuses as short-circuit protection in Low Voltage DC microgrids is evaluated. The main advantage of fuses is that they are simple, cheap, standardized and have low steady state losses. A theoretical basis is formed to model DC short-circuit currents in grids with a limited short-circuit availability. The outcomes are applied to evaluate the possibilities of fuse protection in LVDC grids. It was found that fuses are an effective means of protection, although the required amount of capacitance at the output of the voltage balancing converter can be high, which impacts the total system cost. A fuse based protection strategy is presented that highlights the need for additional capacitance to clear faults compared to the necessary capacitance for system stability. An experimental setup was built to validate the claims.
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