Questions Which clustering algorithms are most effective according to different cluster validity evaluators? Which distance or dissimilarity measure is most suitable for clustering algorithms? Location Hyrcanian forest, Iran (Asia), Virginia region forest, United States (North America), beech forests, Ukraine (Europe). Methods We tested 25 clustering algorithms with nine vegetation data sets comprised of three real data sets and six simulated data sets exhibiting different cluster separation values. The clustering algorithms included both hierarchical and non‐hierarchical partitioning. Five evaluators were employed on each cluster solution to evaluate different clustering algorithms. Algorithms were ranked from best to worst on each clustering evaluator for each data set. Results The comparison revealed that the OPTSIL initiated from a Flexible‐β (−0.25) solution achieved particularly good performance. We also found that Ward's method and Flexible‐β (−0.1) implementations were accurate. K‐means with Hellinger distance was superior to Partitioning Around Medoids (PAM) algorithms. Accordance between distance measures and clustering algorithms was also observed. Bray–Curtis dissimilarity combined with a range of clustering algorithms was successful in most cases. Bray–Curtis dissimilarity proved superior to other distance measures for heterogeneous data sets. Conclusions All in all, the results demonstrate that choosing the most suitable method before clustering is critical for achieving maximally interpretable clusters. The complexity of vegetation data sets makes this issue even more important. The choice of distance measure had more effect than the choice of clustering method on the quality of results. Our results illustrate that OPTSIL Flexible‐β (−0.1) and OPTPART could prove superior to alternative conventional clustering algorithms when internal evaluation criteria are used to optimize clustering.
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