Background: The need for assisted reproduction technologies (ART) for the establishment of pregnancies has steadily increased worldwide. Therefore, it is of vital importance that an efficient sperm preparation technique used for retrieval of high-quality spermatozoa contributes to the creations of high-quality embryos, with high implantation potential. Objective: to study the effect of swim up technique on human sperm motility and DNA integrity. Subject and methods: A prospective study carried on 70 samples of human semen; each sample, divided into 2 parts, one part was prepared by swim-up technique and the other not, and then study sperm motility and DNA integrity in both parts. Sperm DNA integrity was determined using a modified alkaline single cell gel electrophoresis (comet) assay and acridine orange test (AOT), and motility was determined by light microscope. Result(s): the results showed that swim up technique give a significant. increasing in motility percentage and a significant. decreasing in DNA damage (P< 0.05) than unprepared human sperm. Conclusion(s): Swim up technique for sperm preparation is increasing motility and decreasing DNA damage.
Background: Gestational hypertension exerts a great challenge on the maternal cardiovascular system, in spite of this fact, there is lack of reports regarding the maternal diastolic function in gestational hypertension which precedes systolic dysfunction in any cardiovascular complications. Objectives: To evaluate the maternal left ventricular diastolic function in gestational hypertensive women in the third trimester by measuring the mitral inflow parameters with pulse wave Doppler and Tissue Doppler Imaging. Patients and Methods: This studywas conducted in Baghdad teaching hospital from November 2015 to June 2016. Sixty gestational hypertensive womenwith singleton pregnancy in the third trimester, aged 29.7 ± 5.9 year, gestational hypertension was defined as systolicblood pressure that is equal or more than 140 mmHg or diastolic bloodpressurethatequals or exceeds 90 mmHg and starts after 20 weeks of gestation without proteinuria were enrolled in this study. Another 50 Normal pregnant womenaged 28 ± 3.18year served as controls. The left ventricular diastolic function was studied using two transthoracic echocardiographic methods: Pulsed wave Doppler (PWD) to measure the transmitral flow velocity including the early maximum velocity of mitral inflow(E wave), the late maximum velocity of mitral inflow (A wave), and E/A ratio was recorded. Tissue Doppler imaging (TDI) to measure thelateralmitral annular velocity (lateral é), the septal mitral annular velocity (septal é) and their average (é) was calculated. The ratio of E/é was recorded. Results: Gestational hypertensive women hadsignificant lower E wave velocity compared tonormal pregnant women (P value was 0.001). "A"wave velocity was higher in gestational hypertensive women with statistical difference(P value 0.002) and E/A was significant low in gestational hypertension with P value <0.05. Tissue Doppler imaging showed significant difference in é velocity which was lowerin gestational hypertensive women (P value 0.001). E/éin gestational hypertension showed significantly higher value than controls with P value<0.05. Conclusion: Gestational hypertension puts the maternal heart under pressure and volume overload associated with impaired relaxation as manifested by the changes inthe left ventricular diastolic function measured by transthoracic echocardiography.
It is well known that sperm is a unique cell in that it has a function to be done by itself outside the body and this function is essential for species' continuity thus sperm by its power and intact structure has to reach the ova and perform the fertilization and this journey is affected by the chemical and physical factors that might increase or decrease its ability to move or fertilize or even to survive. The aim of this study is to find the effect of vibration that is a vigorous movement with high frequency for 20min on whole seminal fluid samples as an external physical factor. 40 fresh seminal fluid samples were selected. 1ml of each semen samples was placed in the bottom of conical tube; the tube was exposure to vibration waves by using a special shaker designed for this purpose for 20 min. This shaker consist of a M540 DC motor equipped with PWM controller to control the rotational speed from 5-2400 rpm. Semen analysis was done before and after subject vibration. A significant increase (P<0.05) was found in percentage of sperm active directed motility (grade A) with a non-significant increase in sluggish motility and a non-significant decrease in percentage of immotile sperms percentage. No significant changes were founded regarded sperm morphology and count. It was concluded that vibrating seminal sample for 20min increases the overall sperms activity with significant increase in percentage of highly active directed sperms.
During cryopreservation, cells and tissue undergo dramatic transformation in chemical and physical characteristics. Thawing process is also an important step since the cryoinjury is not limited to the freezing process but may also occur during the thawing process. Laser by its photo-stimulation effect can improve the resistance to cooled storage of some human sperm and this will lead to improvement in the quality of frozen semen and in the potential of sperm fertilization. The objective of this study is to use laser as a method of thawing and its effect on human sperm motility and DNA integrity versus the thawing through room temperature. This prospective study carried on 70 cryopreserved semen samples. Each sample was prepared by centrifugation procedure, and divided into 2 parts, freezed and thawed by laser irradiation till melting for one part and by room temperature for other. The DNA integrity and sperm motility were assessed before vitrification and after the two methods of thawing. The results of cryopreserved semen samples showed that laser irradiation thawing has significantly increased sperm motility as well as a significant decreased DNA fragmentation (P< 0.05) versus room temperature thawing. Conclusion(s): Laser irradiation thawing of post freezing sperm improves post thaw motility and DNA integrity.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.