Objectives To study environmental exposure to benzene as well as hematological effects of benzene exposure in taxi drivers. Background Exposure to benzene has been associated with adverse health effects, including haematopoietic disorders. Methods A retrospective cohort study was carried out from the beginning of April 2017, through the end of June 2018, on 280 taxi drivers (the exposed group) and 120 unexposed matched individuals (controls). The tools included a predesigned self-administered questionnaire which contained questions about personal data (e.g., age, educational level, and smoking) and exposure data (e.g., duration and nature of exposure). Investigations involved complete blood count. Environmental studies for light-chain aromatic hydrocarbons (BTEX components: benzene, toluene, ethylbenzene, and xylene) were done. Results Mean values (ppm) of ambient concentrations of benzene, toluene, ethylbenzene, and xylene (0.81 ± 0.42, 26.69 ± 0.54, 29.36 ± 2.35, and 25.11 ± 2.06, respectively) in the stations were higher than international permissible levels (P < 0.001). On studying the clinical manifestations during the last two weeks, the prevalence of pallor, dizziness, headache, waist and back pain, fatigue, dry throat, and discomfort was significantly higher in taxi drivers (27.1%, 24.3%, 21.4%, 28.6%, 45.7%, 24.3%, and 25.7%, respectively) than among the controls (6.7%, 4.2%, 6.7%, 10%, 10%, 6.7%, 6.7%, and 9.2%, respectively). For chronic diseases, hypertension was the most prevalent chronic disease among the drivers (17.1%) than the controls (5.8%) (P=0.002). Regarding self-assessment of health status, 20.0% of taxi drivers reported poor health while 31.4% reported very good health (P < 0.001). MCH (pg), TLC (×103/μl), and platelets (×103/μl) were significantly lower among taxi drivers (26.33 ± 2.31, 6.55 ± 1.38, and 189.07 ± 53.25, respectively) (P=0.005, <0.001, and <0.001 respectively). Conclusion Abnormal hematological findings among taxi drivers were found on exposure to benzene. Health of taxi drivers is generally affected. Setting a clinic for periodic checkup and health education for taxi drivers is highly recommended.