BackgroundDendritic cells (DCs) can take up an array of different antigens, including microorganisms which they can process and present more effectively than any other antigen presenting cell. However, whether the interaction between the human DC and Mycobacterium tuberculosis represents a defense mechanism by the invaded host, or helping the invader to evade the defense mechanism of the host is still not clearly understood.FindingsTo analyze the interactions between M. tuberculosis and immune cells, human peripheral blood monocyte-derived immature DCs were infected with M. tuberculosis H37Rv wild type strain and flow cytometry was used to analyse cell surface expression markers. The ability of the M. tuberculosis infected DC to induce T cell proliferation using 5 and 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) dilution technique was also investigated. DCs were found to internalize the mycobacteria and show dose dependent infection and necrosis with different multiplicity of infection. Flow cytometry analysis of cell surface expression markers CD40, CD54, CD80, CD83, CD86 and HLA DR in infected DC revealed significant (p < 0.05) up regulation following infection with M. tuberculosis in comparison to immature DC with no stimulation. Lipopolysaccharide (LPS) from Salmonella abortus equi, a known DC maturation agent, was used as a positive control and showed a comparable up regulation of cell surface markers as observed with M. tuberculosis infected DC. It was revealed that the M. tuberculosis infected DC induced T cell proliferation.ConclusionThese data clearly demonstrate that M. tuberculosis induces activation and maturation of human monocyte-derived immature DC as well as induces T cell proliferation in vitro.
Background: Bovine trypanosomosis is a serious constraint to agricultural production in extensive areas of Ethiopia. Methodology: A cross-sectional study was conducted to determine the prevalence of bovine infection with trypanosomes and to identify the prevailed trypanosome species in three districts of the East Gojjam zone bordering the Blue Nile River from March 2005 to February 2006. Cattle from 9 different localities were checked using microscopical examination of wet blood smears, thin and stained bloodsmears, and by blood centrifugation followed by the examination of the resultant buffy coats. Result: Of the total 3,360 cattle investigated, 8.2% (3.5%, 11.6% and 9.4% from Dejen, Machakel and Baso-Liben districts respectively) were found to be infected with trypanosomes. Of the total 275 positive animals, 249 (90.5%) appeared to be infected with Trypanosoma vivax; 11 (4%) were infected with T. congolense; and 15 (5.5%) were infected with mixed infection of T. vivax and T. congolense. The prevalence of infection with T. vivax was significantly higher than that of T. congolense (P
This book chapter describes the epidemiological, clinical and pathological features of TB in South American camelids (llamas and alpacas) in Great Britain, as well as the available diagnostic tests and disease control strategies. The extent of mycobacterial diseases in South American camelids caused by M. bovis and other mycobacteria in other countries and reports from Africa and the Middle East on the problem of TB in old world camels are highlighted.
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