Self-assembled nanostructures of peptide amphiphiles (PAs) with molecular structures C 16 K 2 and C 16 K 3 (where C indicates the number of carbon atoms in the alkyl chain and K is the lysine in the head group) were studied by a combination of theoretical modeling, transmission electron and atomic force microscopes, and acid−base titration experiments. The supramolecular morphology of the PAs (micelles, fibers, or lamellas) was dependent on the pH and ionic strength of the solution. Theoretical modeling was performed using a molecular theory that allows determining the equilibrium morphology, the size, and the charge of the soft nanoassemblies as a function of the molecular structure of the PA, and the pH and salt concentration of the solution. Theoretical predictions showed good agreement with experimental data for the pH-dependent morphology and size of the nanoassemblies and their apparent pK a s. Two interesting effects associated with charge regulation mechanisms were found: first, ionic strength plays a dual role in the modulation of the electrostatic interactions in the system, which leads to complex dependencies of the aggregation numbers with salt concentration; second, the aggregation number of the nanostructures decreases upon increasing the charge per PA. The second mechanism, charge regulation by size regulation, tunes the net charge of the assemblies to decrease the electrostatic repulsions. A remarkable consequence of this behavior is that adding an extra lysine residue to the charged region of the PAs can lead to an unexpected decrease in the total charge of the micelles. 59 Antimicrobial properties are also highly dependent on the 60 charge of the PAs: a recent study has shown that cationic PAs 61 can inhibit the formation of bacterial films, while anionic ones 62 show no antimicrobial activity at all. 25 In a related biomedical 63 application, the performance of vaccines prepared from PA 64 nanostructures was found to be strongly dependent on their 65 morphology, size, and charge. 26 The importance of nanostruc-66 ture morphology and charge transcends the biological uses of 67 PAs and spans nanotechnology applications as well. For 68 example, Stupp's group has developed a biomineralization 69 strategy for PA nanofibers that requires a negative surface
We present molecular dynamics simulation results pertaining to the solvation of Li(+) in dimethyl sulfoxide-acetonitrile binary mixtures. The results are potentially relevant in the design of Li-air batteries that rely on aprotic mixtures as solvent media. To analyze effects derived from differences in ionic size and charge sign, the solvation of Li(+) is compared to the ones observed for infinitely diluted K(+) and Cl(-) species, in similar solutions. At all compositions, the cations are preferentially solvated by dimethyl sulfoxide. Contrasting, the first solvation shell of Cl(-) shows a gradual modification in its composition, which varies linearly with the global concentrations of the two solvents in the mixtures. Moreover, the energetics of the solvation, described in terms of the corresponding solute-solvent coupling, presents a clear non-ideal concentration dependence. Similar nonlinear trends were found for the stabilization of different ionic species in solution, compared to the ones exhibited by their electrically neutral counterparts. These tendencies account for the characteristics of the free energy associated to the stabilization of Li(+)Cl(-), contact-ion-pairs in these solutions. Ionic transport is also analyzed. Dynamical results show concentration trends similar to those recently obtained from direct experimental measurements.
A molecular theory is introduced to model the layer-by-layer self-assembly (LbL-SA) of polymers with pairing interactions. Our theory provides a general framework to describe nonelectrostatic LbL-SA as the pairing interactions generically describe the formation of bonds between two complementary chemical species, for example, hydrogen donor and acceptor in hydrogen-bonding-LbL or host and guest in host-guest-LbL. The theory predicts fundamental observations related to LbL-SA: (i) phase separation of a mixture of polymers with pairing interactions in bulk solution, (ii) linear increase in film thickness with the number of LbL adsorption steps, (iii) stoichiometry overcompensation after each adsorption step, and (iv) interpenetration of polymer layers. Importantly, this study shows that the minimal requirement for nonelectrostatic LbL is the competition of a pairing interaction and an excluded-volume repulsion. A simple analytical model based on this competition predicts the volume fraction of the layers in good agreement with the numerical predictions of the molecular theory.
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