Summary1. Endangered species subjected to reintroduction programmes often occur as small and isolated populations with local high density and depressed fecundity. Variation in territory quality may lead to this low fecundity owing to increasing occupation of suboptimal territories as population density grows, known as the habitat heterogeneity hypothesis (HHH). In this context, food supplementation in poor territories may be used to produce extra young which could be allocated to reintroduction programmes. 2. We analyse the density-dependent fecundity pattern and the underlying mechanism in a small population of bearded vultures Gypaetus barbatus in Arag on (northeast Spain). We then use population simulations to examine the viability of a hypothetical reintroduction programme using extra young produced by supplementary feeding on poor-quality territories and the effect on the donor population. We also compare the economic cost of such a reintroduction programme in relation to the cost of a traditional captive breeding programme. 3. The wild population showed clear negative, density-dependent fecundity regulation driven by the HHH mechanism. Simulations showed that extractions for translocations had no relevant long-term effects on the donor population viability, but a marked population reduction during the extraction period. However, the implementation of supplementary feeding to produce extra young for translocation lessened significantly this expected initial population reduction. 4. Analyses showed that the annual budget of a captive breeding programme for this species could be seven times more expensive than the translocation of extra young produced by food supplementation. 5. Synthesis and applications. Reintroduction programmes based on translocation of wildreared individuals, after a supplementary feeding programme oriented to poor-quality territories, provide a source of young at least seven times cheaper than those from captive breeding programmes. The use of this approach would decrease initial effects on donor population avoiding public criticism. Increasing the number of young released during the first years of the reintroduction decreases total financial cost and increases the final population size in the new area.

The objective of this study was to determine the prevalence and diversity of coagulase-negative staphylococci (CoNS) species from wild birds in Spain, as well as to analyze the antimicrobial resistance phenotype/genotype and the virulence gene content. During 2015–2016, tracheal samples of 242 wild birds were collected in different regions of Spain for staphylococci recovery. The species identification was performed using MALDI-TOF. The antimicrobial resistance phenotype and genotype was investigated by the disk diffusion method and by PCR, respectively. The presence of the virulence genes lukF/S-PV, tst, eta, etb, etd and scn was investigated by PCR. Moreover, CoNS carrying the mecA gene were subjected to SCCmec typing. Of the tested animals, 60% were CoNS-carriers, and 173 CoNS isolates were recovered from the 146 positive animals, which belonged to 11 species, with predominance of S. sciuri (n = 118) and S. lentus (n = 25). A total of 34% of CoNS isolates showed a multidrug resistance phenotype, and 42 mecA-positive methicillin-resistant CoNS (MRCoNS) were detected. The isolates showed resistance to the following antimicrobials (percentage of resistant isolates/antimicrobial resistance genes detected): penicillin (49/ blaZ, mecA), cefoxitin (24/ mecA), erythromycin and/or clindamycin (92/ erm(B), erm(C), erm(43), msr(A), mph(C), lnu(A), lsa(B), vga(A) and sal(A)), gentamicin and/or tobramycin (5/ aac(6′)-Ie-aph(2″)-Ia, ant(4′)-Ia), streptomycin (12/str), tetracycline (17/ tet(K), tet(L), tet(M)), ciprofloxacin (4), chloramphenicol (1/ fexA), fusidic acid (86/ fusB, fusD) and trimethoprim–sulfamethoxazole (1/ dfrK). None of the isolates harbored the lukF/S-PV, eta, etb, etd and scn genes, but two S. sciuri isolates (1%) carried the tst gene. Wild birds are frequently colonized by CoNS species, especially S. sciuri. We identified scavenging on intensively produced livestock and feeding on landfills as risk factors for CoNS carriage. High proportions of MRCoNS and multidrug resistant CoNS were detected, which coupled with the presence of important virulence genes is of concern.

The NERC and CEH trademarks and logos ('the Trademarks') are registered trademarks of NERC in the UK and other countries, and may not be used without the prior written consent of the Trademark owner. Accepted ArticleThis article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Accepted ArticleThis article is protected by copyright. All rights reserved. 3. Results of Generalised linear mixed model (GLMM) analysis with relative productivity as the dependent variable, species and supplementary feeding as fixed factors and territory as random factor, showed a significant effect of supplementary feeding on relative productivity in both species as well as in the interaction between territory and supplementary feeding. This implied a different response among territories to supplementary feeding. Birds in poor quality territories with low productivity levels responded more strongly to supplementary feeding than birds in territories with higher levels of natural productivity.4. A reintroduction programme based on supplementary feeding and extraction of nestlings costs eight times less than the same program based on captive breeding, and takes ten years less.5. Synthesis and applications. Supplementary feeding in territorial raptors could be useful in two situations: (i) in an episodic main prey collapse and (ii) in poor quality territories in a high density population, to produce extra young for reintroduction programmes. For greatest efficiency, supplementary feeding needs to be targeted at the poorer territories in which the reproductive rate has the potential to be raised by provision of extra food. The extra young produced can then be used in reintroduction programmes in which their chances of recruiting to a breeding population are high.

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