Different cultivars of four Vaccinium species [Vaccinium
corymbosum L (Highbush), Vaccinium
ashei Reade (Rabbiteye), Vaccinium angustifolium (Lowbush), and Vaccinium myrtillus L (Bilberry)]
were analyzed for total phenolics, total anthocyanins, and antioxidant capacity (oxygen radical
absorbance capacity, ORAC). The total antioxidant capacity of different berries studied ranged
from a low of 13.9 to 45.9 μmol Trolox equivalents (TE)/g of fresh berry (63.2−282.3 μmol TE/g of
dry matter) in different species and cultivars of Vaccinium. Brightwell and Tifblue cultivars of
rabbiteye blueberries were harvested at 2 times, 49 days apart. Increased maturity at harvest
increased the ORAC, the anthocyanin, and the total phenolic content. The growing location (Oregon
vs Michigan vs New Jersey) did not affect ORAC, anthocyanin or total phenolic content of the cv.
Jersey of highbush blueberries. A linear relationship existed between ORAC and anthocyanin (r
xy
= 0.77) or total phenolic (r
xy
= 0.92) content. In general, blueberries are one of the richest sources
of antioxidant phytonutrients of the fresh fruits and vegetables we have studied.
Keywords: Vitamin C; ascorbate; blueberry; bilberry; highbush; lowbush; rabbiteye; ORAC; HPLC
Blueberries and blackberries grown at various locations in Georgia were collected and analyzed for flavonoids, total anthocyanins, total polyphenols, and Trolox-equivalent antioxidant capacity (TEAC). Each sample was analyzed for phenolic acids (gallic acid, p-hydroxybenzoic acid, caffeic acid, ferulic acid, and ellagic acid) and flavonoids (catechin, epicatechin, myricetin, quercetin, and kaempferol). A high-performance liquid chromatographic (HPLC) method with photodiode array detection was used for analysis. Compounds were analyzed as aglycons after acid hydrolysis with 1.2 M HCl. Identification of each compound was based on retention time and UV spectra by comparison with pure commercial standards. Phenolic acids ranged from 0.19 to 258.90 mg/100 g fresh weight (FW), and flavonoids ranged from 2.50 to 387.48 mg/100 g FW. Total polyphenols ranged from 261.95 to 929.62 mg/100 g FW, and total anthocyanins ranged from 12.70 to 197.34 mg/100 g FW. TEAC values varied from 8.11 to a maximum of 38.29 microM/g FW. A linear relationship was observed between TEAC values and total polyphenols or total anthocyanins. The data indicate that blueberries and blackberries are rich sources of antioxidants.
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