In patients undergoing cardiac surgery, transfusion of red cells that had been stored for more than 2 weeks was associated with a significantly increased risk of postoperative complications as well as reduced short-term and long-term survival.
This report describes the important issues considered by the CAP committee during the development of the new checklist requirements, which address documentation, validation, quality assurance, confirmatory testing, exception logs, monitoring of upgrades, variant interpretation and reporting, incidental findings, data storage, version traceability, and data transfer confidentiality.
Chronic lymphocytic leukemia (CLL) is well characterized clinically and immunophenotypically. Demonstration of a monotypic CD19+, CD5+ B-cell population is central to the diagnosis. We report 2 cases of biclonal CLL. Two elderly men were encountered with an absolute lymphocytosis consisting of the typical CD5+, CD19+, CD23+ B-cell population seen in CLL; however, immunoglobulin light chain restriction by flow cytometry was not apparent as B cells expressed kappa or lambda light chains without a clear monotypic population. Molecular genetic analysis of flow cytometry-sorted cells (kappa and lambda populations) revealed in both cases 2 monoclonal B-cell populations. The characterization of these cases and a review of the issues surrounding biclonal CLL are presented.
Rearrangements or deletions of chromosome 17 are the most frequently observed genetic changes identified in breast tumors. Molecular analyses suggest that in addition to the p53 gene on 17p13.1 there may be at least three other tumor suppressor genes on chromosome 17 involved in breast cancer. Regions of loss of heterozygosity (LOH) identified on 17p13.3 and 17q12-qter occur frequently in breast tumors, and the BRCA-1 gene has been mapped to 17q21 by genetic linkage analysis. Here we provide biological evidence for the presence of a growth suppressor gene(s) on chromosome 17 that results in the in vitro growth suppression of the p53 wild-type MCF 7 breast cancer cell line. We have introduced a normal chromosome 17 into MCF 7 cells by microcell-mediated chromosome transfer (MMCT), and demonstrate that cells growth arrest before 10 to 12 population doublings. In contrast, the introduction of a normal chromosome 13 had no effect upon growth of these cells either in vitro or in vivo. These data provide direct functional evidence for the presence of a growth suppressor gene(s) on chromosome 17, which is not p53, and which may represent one of several gene(s) that play a critical role in the development of breast cancer.
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