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An efficient preparation of N‐succinimidyl 4‐[18F]fluorobenzoate ([18F]SFB) based on a convenient three‐step, one‐pot procedure is described. [18F]Fluorination of the precursor ethyl 4‐(trimethylammonium triflate)benzoate gave ethyl 4‐[18F]fluorobenzoate. Saponification of the ethyl 4‐[18F]fluorobenzoate with aqueous tetrapropylammonium hydroxide yielded the corresponding 4‐[18F]fluorobenzoate salt ([18F]FBA), which was then treated with N,N,N,N′‐tetramethyl‐O‐(N‐succinimidyl)uronium hexafluorophosphate. The purified [18F]SFB was used for the labeling of Avastin™ (Bevacizumab) through [18F]fluorobenzoylation of the Avastin's α‐amino groups. The decay‐corrected radiochemical yields of [18F]SFB were as high as 44% (based on [18F]fluoride (n=10) with a synthesis time of less than 60 min. [18F]Avastin was produced in decay‐corrected radiochemical yields of up to 42% (n=5) within 30 min (based on [18F]SFB). The radiochemical purities of [18F]SFB and [18F]Avastin were greater than 95%. Copyright © 2008 John Wiley & Sons, Ltd.

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